摘要
目的 分析男性解脲支原体(Uu)感染对精液常规参数、精子功能形态及辅助生殖妊娠结局的影响。方法 回顾性分析2020年1月至2020年12月在该院门诊接受精液分析的22 265例不育男性患者的病历资料。采用Uu试剂盒检测Uu感染情况,根据是否存在Uu感染分为Uu阴性组与Uu阳性组。采集两组精液样本,检测并比较两组精子密度、精液量、液化时间、精子存活率、精子活力(a-b)、直线速度(VSL)、曲线速度(VCL)、平均路径(VAP)、精子DNA碎片、精子核蛋白组型。随访至2023年5月,记录并比较两组受精率、流产率、早产率。结果 Uu阴性组共18 793例,Uu阳性组共3 472例,两组精液量比较,差异无统计学意义(P>0.05);Uu阳性组精子密度、精子活率均明显低于Uu阴性组,液化时间明显高于Uu阴性组,差异均有统计学意义(P<0.05)。Uu阳性组精子畸形率、DNA碎片、核蛋白质组型均明显高于Uu阴性组,差异均有统计学意义(P<0.05)。两组a-b、VAP、VCL水平比较,差异均无统计学意义(P>0.05);Uu阳性组VSL水平明显低于Uu阴性组,差异有统计学意义(P<0.05)。16 651例接受辅助生殖技术助孕治疗的患者中,Uu阴性组14 287例,Uu阳性组2 364例。Uu阳性组受精率低于Uu阴性组,流产率、早产率均高于Uu阴性组,但差异均无统计学意义(P>0.05)。结论 Uu感染可导致男性患者精液密度、活力下降,增加液体液化时间及精子畸形率,影响精液前进运动能力及功能,但对辅助生殖妊娠结局影响较小。
Objective To analyze the influence of Ureaplasma urealyticum(Uu) infection on routine semen parameters,sperm function and morphology,and pregnancy outcome in assisted reproductive technology.Methods The medical records of 22 265 infertile male patients who underwent semen analysis in the outpatient department of the hospital from January 2020 to December 2020 were retrospectively analyzed.Uu infection was detected by Uu kit,and the patients were divided into Uu negative group and Uu positive group according to the presence or absence of Uu infection.Semen samples were collected from the two groups,and sperm density,semen volume,liquefaction time,sperm viability,sperm motility(a-b),linear velocity(VSL),curved velocity(VCL),average path(VAP),sperm DNA fragmentation,sperm nucleoproteome were detected and compared between the two groups.The patients were followed up until May 2023,and the fertilization rate and abortion rate,preterm birth rate of the two groups were recorded and compared.Results There were 18 793 cases in Uu negative group and 3 472 cases in Uu positive group.There was no significant difference in semen volume between the two groups(P>0.05).The sperm density and sperm motility in the Uu positive group were significantly lower than those in the Uu negative group,and the liquefaction time in the Uu positive group was significantly longer than that in the Uu negative group,and the difference was statistically significant(P<0.05).The rate of sperm malformation,DNA fragmentation,and nuclear proteomics in the Uu positive group were significantly higher than those in the Uu negative group(P<0.05).There was no statistically significant difference in the levels of a-b,VAP and VCL between the two groups(P>0.05).The level of VSL in Uu positive group was significantly lower than that in Uu negative group(P<0.05).Among 16 651 patients who received ART,14 287 cases were Uu negative and 2 364 cases were Uu positive.The fertilization rate of the Uu positive group was lower than that of the Uu negative group,and the
作者
寇卉
石绒
杨杰
苏婧
张洲
KOU Hui;SHI Rong;YANG Jie;SU Jing;ZHANG Zhou(Department of Reproductive Center,Northwest Women and Children's Hospital,Xi'an,Shaanxi 710004,China)
出处
《检验医学与临床》
2024年第5期654-657,共4页
Laboratory Medicine and Clinic
关键词
男性
解脲支原体
精液
功能
形态
生殖妊娠
male
ureaplasma urealyticum
semen
function
morphology
reproductive pregnancy
