摘要
目的建立测定藏药亚吉玛(肉质金腰)中金腰乙素含量的高效液相色谱法。方法色谱柱为Inertsustain C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.2%磷酸溶液(40∶60,V/V),流速为1.2 mL/min,检测波长为350 nm,柱温为35℃,进样量为10μL。结果金腰乙素的进样量在0.04~5μg范围内与峰面积线性关系良好(R2=0.9990,n=8);精密度、稳定性、重复性试验结果的RSD均小于2.0%(n=6);平均加样回收率为95.39%,RSD为1.64%(n=6)。样品中金腰乙素含量为0.1323%~0.6127%(n=3)。结论所建立的方法结果准确,精密度、重复性及耐用性均良好,可用于肉质金腰中金腰乙素的含量测定。
Objective To establish a high-performance liquid chromatography(HPLC)method for the content determination of chrysosplenetin in Tibetan medicine Yajima(Chrysosplenium carnosum).Methods The chromatographic column was Inertsustain C18 column(250 mm×4.6 mm,5μm),the mobile phase was acetonitrile-0.2%phosphoric acid solution(40∶60,V/V),the flow rate was 1.2 mL/min,the detection wavelength was 350 nm,the column temperature was 35℃,and the injection volume was 10μL.Results The linear range of chrysosplenetin was 0.04-5μg(R2=0.9990,n=8).The RSDs of precision,stability,and repeatability test results were all lower than 2.0%(n=6).The average recovery of chrysosplenetin was 95.39%with an RSD of 1.64%(n=6).The content of chrysosplenetin in samples was in the range of 0.1323%-0.6127%(n=3).Conclusion The established method is accurate,with good precision,repeatability,and durability,which can be used for the content determination of chrysosplenetin in Chrysosplenium carnosum.
作者
郭丽萍
次旦多吉
任刚
邱毅
尼珍
GUO Liping;Cidanduoji;REN Gang;QIU Yi;Nizhen(Xizang Institutes for Food and Drug Control·NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine<Tibetan Medicine>·Xizang Key Laboratory of Standardization Research in Tibetan Medicine,Lhasa,Xizang,China 850000;Jiangxi University of Chinese Medicine·Research Center of Natural Resources of Chinese Medicinal Materials and Ethnic Medicine,Nanchang,Jiangxi,China 330004)
出处
《中国药业》
CAS
2024年第5期83-86,共4页
China Pharmaceuticals
基金
国家药典委员会国家药品标准提高研究项目[411]。
