摘要
围绕金银花新品种“华金6号”长蕾期表型,以“华金6号”及“大毛花”为材料,探究其表型形成机制。检测内源茉莉酸类植物激素(jasmonic acid hormones,JAs)含量;对忍冬进行转录组分析,确定与茉莉酸(jasmonic acid,JA)合成相关基因;采集不同时期“华金6号”及“大毛花”的花蕾及花,利用qRT-PCR(quantitative real-time PCR)技术,分析合成相关酶基因在金银花花蕾期表达量的变化趋势。研究发现“华金6号”金银花中JAs含量显著低于“大毛花”;对“华金6号”喷施外源茉莉酸甲酯(methyl-jasmonate,MeJA)可以恢复其开花表型,使其趋近于“大毛花”;2个丙二烯氧化物合酶基因(allene oxide synthase,AOS)、3个脂氧合酶基因(lipoxygenase,LOX)及2个丙二烯环氧化酶基因(allene oxide cyclase,AOC)在“华金6号”和“大毛花”不同时期花及花蕾中的表达量具有显著差异。推测JA合成相关酶基因在“华金6号”中的低表达导致JA合成受阻,从而引起长蕾期表型的形成。本研究为忍冬的遗传育种奠定了一定的基础,为忍冬品种改良提供新思路,为JAs在植物花器官方面的研究提供参考。
Based on the long bud stage phenotype of a new Lonicera japonica Flos variety"Huajin 6",using"Huajin 6"and"Da Mao Hua"as materials,probing the mechanism of its phenotype formation.Detection of endogenous Jasmonic acid hormones(JAs)content;the genes related to jasmonic acid(JA)synthesis were identified by transcriptome analysis of Lonicera japonica;flower buds and flowers of"Huajin 6"and"Da Mao Hua"were collected at different periods,and the qRT-PCR(quantitative real-time PCR)technique was used to analyze the trend of the expression of synthesis-related enzyme genes in Lonicera japonica Flos during the bud stage.The study found that the content of JAs in"Huajin 6"Lonicera japonica Flos was significantly lower than that in"Da Mao Hua";applying exogenous methyl-jasmonate(MeJA)to"Huajin 6"can restore its flowering phenotype,making it close to wild type Lonicera japonica Flos;there are significant differences in the expression of two allene oxide synthase genes(AOS),three lipoxygenase genes(LOX),and two allene oxide cyclase genes(AOC)in the flowers and buds of"Huajin 6"and"Da Mao Hua"at different periods.It is hypothesized that the low expression of JA synthesis-related enzyme genes in"Huajin 6"leads to the blockage of JA synthesis,which causes the formation of the long bud phenotype.This study laid a certain foundation for the genetic breeding of Lonicera japonica,provided a new idea for the improvement of Lonicera japonica varieties,and provided a reference for the study of JAs in plant flower organs.
作者
李润竹
梁从莲
刘振华
李佳
张永清
刘海燕
蒲高斌
LI Run-zhu;LIANG Cong-lian;LIU Zhen-hua;LI Jia;ZHANG Yong-qing;LIU Hai-yan;PU Gao-bin(Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Shandong Provincial Collaborative Innovation Center for Quality Control and Construction of the Whole Industrial Chain of Traditional Chinese Medicine,Jinan 250355,China;Forestry Development Center of Pingyi County,Linyi 273300,China)
出处
《药学学报》
CAS
CSCD
北大核心
2024年第2期476-481,共6页
Acta Pharmaceutica Sinica
基金
山东省重点研发计划(乡村振兴科技创新提振行动计划)项目(2022TZXD0036)
山东省农业良种工程项目(2021LZGC008)
山东省现代农业产业技术体系中草药创新团队项目(SDAIT-20)
。
关键词
华金6号
金银花
茉莉酸
生物合成
Huajin 6
Lonicera japonica Flos
jasmonic acid
biosynthesis
