摘要
目的:从VEGF/VEGFR2/Dock6信号途径探索运动预处理对脑缺血再灌注损伤大鼠缺血侧脑组织血管新生的影响。方法:采用随机数字表法将SD雄性大鼠分为假手术组、模型组和运动预处理组,每组18只。造模前,假手术组与模型组不给予任何处理,运动预处理组大鼠给予适应性跑步训练3d,电动跑台坡度为0°,速度10m/min,每天1次,每次20min。适应性训练结束后,运动预处理组给予为期3周的正式跑步训练,每周连续训练6d,休息1d,电动跑台坡度为0°,速度15m/min,30min/d。模型组和运动预处理组采用Koizumi栓线法加以改良制备大鼠大脑中动脉栓塞(MCAO)模型,假手术组仅切开皮肤,不插栓线。采用Zea-Longa评分和改良神经严重程度评分(mNSS)法进行神经功能缺损评估;TTC染色法检测脑梗死体积百分比,HE染色观察缺血侧大脑皮质组织形态学改变;免疫组化法观察缺血侧大脑皮质CD31表达水平;蛋白质免疫印迹测定VEGFA、VEGFR2、Dock6蛋白表达水平。结果:①Zea-Longa评分:再灌注麻醉清醒后,与假手术组相比,其余2组大鼠Zea-Longa评分均增高(P<0.01),且2组间Zea-Longa评分无显著性意义;再灌注72h后,与假手术组相比,模型组大鼠Zea-Longa评分显著增高(P<0.01);与模型组相比,运动预处理组大鼠Zea-Longa评分显著降低(P<0.05)。②mNSS评分:再灌注72h后,与假手术组相比,模型组大鼠mNSS评分显著增高(P<0.01);与模型组相比,运动预处理组大鼠mNSS评分显著降低。③TTC染色:与假手术相比,模型组脑梗死体积百分比增大(P<0.01);与模型组相比,运动预处理组脑梗死体积百分比相对减小(P<0.05)。④HE染色:与假手术组相比,模型组大鼠缺血侧大脑皮质出现显著病理学改变;与模型组相比,运动预处理组大鼠缺血侧大脑皮质病理学改变减轻。⑤CD31免疫组化:与假手术组相比,模型组大鼠缺血侧大脑皮质中CD31(P<0.01)显著升高;与模型组相比,运动预处
Objective:To investigate the effect of exercise preconditioning on angiogenesis in ischemic brain tissue in rats with cerebral ischemia-reperfusion injury in the view of VEGF/VEGFR2/dock6 signaling pathway Method:SD male rats were divided into sham group,model group and exercise preconditioning group by random number table method,with 18 rats in each group.The sham operation group and the model group were not given any treatment,while the exercise preconditioning group was given adaptive running training for 3 days at a speed of 10 m/min,once a day for 20 minutes each time.After the adaptive training,the exercise preconditioning group was given formal running training for 3 weeks,continuous training for 6 days a week,rest for 1 day,electric treadmill slope of 0°,speed of 15m/min,30min/d.Model group and exercise preconditioning group were modified to prepare the middle cerebal artery occlusion(MACO)models by Koizumi thread method,while sham operation group only given skin cutting without thread insertion.Zea longa score and modified neurological severity score(mNSS)were used to score neurological deficit in rats,the relative infarct size of the brain was detected by TTC staining,the morphological changes of the ischemic cerebral cortex was observed by HE staining,the expression of CD31 in ischemic cerebral cortex was detected by immunohistochemistry and the expressions of VEGFA,VEGFR2,Dock6 in ischemic cerebral cortex were detected by western blot.Result:①Zea-Longa scoring:after awaking from anesthetizati,compared with the sham group,the Zea-Longa scores of the other two groups were increased(P<0.01),and there was no statistical significance in the Zea-Lon⁃ga scores between the two groups.At 72 hours after reperfusion,compared with the sham group,the Zea-Longa score of the rats in the model group was significantly increased(P<0.01);compared with the model group,the Zea-Longa score of the rats in the exercise preconditioning group was significantly decreased(P<0.05).②mNSS scoring:At 72 hours after reperfusio
作者
孙晓莹
周璐
唐丽亚
刘锦灿
蒋心如
瞿启睿
艾坤
SUN Xiaoying;ZHOU Lu;TANG Liya(Hunan University of Chinese Medicine,Changsha,Hunan,410208)
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2024年第2期170-177,共8页
Chinese Journal of Rehabilitation Medicine
基金
湖南省自然科学基金青年项目(2021JJ40007)
湖南中医药大学研究生科研创新项目(2022CX102)。
关键词
缺血再灌注
运动预处理
血管新生
血管内皮生长因子
血管内皮生长因子受体-2
细胞分裂蛋白6
ischemia-reperfusion
exercise preconditioning
angiogenesis
vascular endothelial growth factor
vascular endothelial growth factor receptors
dedicator of cytokinesis 6
