摘要
目的:探究激素性股骨头坏死(SANFH)骨组织微小核糖核酸(miRs)差异表达谱。方法:选取2019年6月至2023年1月因激素性股骨头坏死和股骨颈骨折行全髋关节置换术的患者各31例,分别记为激素性股骨头坏死组和对照组。采用Illumina高通量测序技术对激素性股骨头坏死患者和股骨颈骨折患者骨组织进行测序,筛选组间差异微小核糖核酸,利用生物信息学方法分析两类人群基因表达谱的差异。结果:通过Illumina高通测序,比对到1668个已知微小核糖核酸,预测到107个新的微小核糖核酸。与对照组比,激素性股骨头坏死组患者骨组织中有76个差异表达的微小核糖核酸(log2(FoldChange)>1.5,P<0.05),其中表达上调的微小核糖核酸有40个,表达下调的微小核糖核酸有36个。Go分析显示,两组差异表达的微小核糖核酸调控的靶基因功能主要包括细胞代谢过程、细胞内信号转导、间充质细胞增殖、细胞杀伤、受体结合、蛋白活性、酶调节活性、解剖结构发育等;京都基因和基因组数据库(KEGG)通路富集分析显示,两组差异表达的微小核糖核酸调控的信号通路主要包括过氧化物酶体增殖物激活受体γ(PPARγ)信号通路、Janus激酶(JAK)/信号转导转录激活因子(STAT)信号通路、转化生长因子-β1(TGF-β1)/Smad3途径、磷脂酰肌醇3激酶(P13K)/丝氨酸苏氨酸激酶(Akt)信号通路、T细胞受体信号通路、血管内皮生长因子(VEGF)信号通路和泛素蛋白酶体途径等。结论:激素性股骨头坏死骨组织中存在差异表达的微小核糖核酸,差异表达的微小核糖核酸可通过作用于PPARγ、JAK/STAT、TGF-β1/Smad3、P13K/Akt等信号通路,参与激素性股骨头坏死的发生发展。
Objective:To investigate the differential expression profile of micrornas(miRs)in bone tissue of steroid-induced avascular necrosis of the femoral head(SANFH).Methods:31 patients who underwent total hip arthroplasty due to SANFH and tibia fractures from June 2019 to January 2023 were selected and recorded as SANFH group and control group respectively.The Illumina high-throughput sequencing technology was used to sequence the bone tissues of SANFH patients and femoral neck fracture patients,and the miRs differences between groups were screened.Bioinformatics was used to analyze the difference of gene expression profiles between the two populations.Results:Through Illumina high pass sequencing,1668 known miRNAs were compared and 107 new miRNAs were predicted.Compared with the control group,there were 76 differentially expressed miRNAs(log2(FoldChange)>1.5,P<0.05)in bone tissue of patients with SANFH,including 40 up-regulated miRNAs and 36 down-regulated miRNAs.Target gene prediction was conducted for 61 differentially expressed miRs.Go analysis showed that the target gene functions regulated by the two groups of differentially expressed miRs mainly include:cell metabolic process,intracellular signal transduction,mesenchymal cell proliferation,cell killing,receptor binding,protein activity,enzyme regulation activity,anatomical structure development,etc.The enrichment analysis of Kyoto encyclopedia of genes and genomes(KEGG)pathway showed that the signal pathways regulated by the two groups of differentially expressed miRs mainly included:peroxisome proliferator activated receptor γ(PPARγ)signal pathway,Janus kinase(JAK)/signal transducer activator of transcription(STAT)signal pathway,transforming growth factor-β1(TGF-β1)/Smad3 pathway,phosphatidylinositol 3 kinase(P13K)/serine threonine protein kinase(Akt)signal pathway,T cell receptor signal pathway,vascular endothelial growth factor(VEGF)signal pathway,ubiquitin proteasome pathway,etc.Conclusion:There are differentially expressed miRs in SANFH bone tissue,whic
作者
胥彪
赖超超
XU Biao;LAI Chaochao(Department of Orthopedics,Dazhou Hospital of Integrated Traditional Chinese and Western Medicine,Dazhou 635000,Sichuan China)
出处
《中国中医骨伤科杂志》
CAS
2024年第1期59-64,共6页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
关键词
高通量测序技术
激素性股骨头坏死
骨组织
微小核糖核酸
生物信息学
high throughput sequencing technology
steroid induced avascular necrosis of femoral head
bone tissue
micro ribonucleic acid
bioinformatics
