摘要
建立了紫外分光光度法测试游离透明质酸钠含量的方法。由于游离透明质酸钠易溶于水,因此其应用受到限制,而采用交联剂合成的交联透明质酸钠,克服了天然透明质酸钠的缺陷,但是难于在水溶液中分散,需要溶解后才能将交联网络中的其他水溶性物质释放出来,从而导致游离透明质酸钠含量的测量有效性降低。在已有研究方法的基础上,通过改变溶解方式和过滤模式,大大改进了测量结果。在0~50μg/mL范围内,样品管中透明质酸钠含量与吸光度的线性较好,相关系数为0.99982;最低检测限为0.008μg/mL;定量限为0.025μg/mL;精密度测试,RSD为0.02%(n=10);重复性测试,RSD为4.3%(n=10);平均加标回收率为94%(n=10)。该测量方法具有准确度高、稳定性好、灵敏度高、重复性好等优点,专属性和适用性更强,可用于测量各种类型的交联透明质酸钠凝胶产品中的游离透明质酸钠含量。
A method for the determination of free sodium hyaluronate by ultraviolet spectrophotometry was established.The application of free sodium hyaluronate is limited due to its solubility in water.The use of crosslinking agents to synthesize crosslinked sodium hyaluronate overcomes the defects of natural sodium hyaluronate,but it is difficult to disperse in aqueous solution,and other water-soluble substances in the crosslinked network need to be dissolved before they can be released,thus reducing the effectiveness of measuring the content of free sodium hyaluronate.On the basis of the existing research methods,the measurement results are greatly improved by changing the dissolution mode and filtration mode.In the range of 0~50μg/mL,the linear relationship between sodium hyaluronate content and absorbance was good,and the correlation coefficient was 0.99982.The minimum detection limit was 0.008μg/mL.The limit of quantification was 0.025μg/mL.For precision test,RSD was 0.02%(n=10);Repeatability test,RSD was 4.3%(n=10);The average recovery rate was 94%(n=10).The method has the advantages of high accuracy,good stability,high sensitivity and good repeatability.And it has more specific and applicable.It can be used to measure the content of free sodium hyaluronate in various types of crosslinked sodium hyaluronate gel products.
作者
朱吉
王娜
耿袁
顾颖涛
石磊
Zhu Ji;Wang Na;Geng Yuan;Gu Ying-tao;Shi Lei(Jiangsu Yilutong Inspection and Testing Technology Service Co.,Ltd.,Zhangjiagang Jiangsu 215600,China)
出处
《江西化工》
CAS
2024年第1期23-26,共4页
Jiangxi Chemical Industry
关键词
透明质酸钠
交联透明质酸钠
游离透明质酸钠
交联剂
紫外分光光度计
回收率
sodium hyaluronate
crosslinked sodium hyaluronate
free sodium hyaluronate
crosslinking agent
ultraviolet spectrophotometer
recovery
