摘要
在深海原位环境下精确捕获和收集单个目标微生物是当前深海微生物研究中的一大挑战。设计了一个应用在深海环境中的光镊捕获系统方案,系统包含光镊模块、微流控芯片、耐压外罩等部分,可实现原位捕获收集深海微生物。建立了位于南海北纬18°附近,1.5 km深处的海水折射率模型,预估了深海对光镊系统激光功率的损耗。使用T矩阵法计算对比了光镊在深海中和陆地上的纯水中对不同折射率和不同大小的球形微生物的捕获力。结果表明,相比于纯水中,光镊在1.5 km的深海中的最大轴向捕获力平均降低了25%,最大横向捕获力平均降低了20%。本研究可为未来基于光镊技术开发的深海微生物分选设备的下海实验提供参考。
A significant hurdle in current deep-sea microbial research remains the inability to capture these microbes in their natural habitat.Optical tweezers,with their non-contact,low-damage,and highly precise capabilities,present an ideal solution for capturing and manipulating microorganisms in liquid environments,showing promise in capturing deep-sea microbes.However,existing research on optical tweezers primarily focuses on ideal mediums such as pure water and air,with few studies exploring their application and the alteration of trapping force characteristics in the deep-sea environment.In order to address the challenge of accurately capturing and collecting deep-sea microorganisms,this paper presents a designed optical tweezers acquisition system tailored for deep-sea environments.The system incorporates an optical tweezers module,a microfluidic chip,and a pressure-resistant cover,facilitating the in-situ capture and sorting of deep-sea microorganisms.In examining the alteration of trapping force in optical tweezers in deep-sea conditions compared to pure water,this study initially outlines the principle and methodology for calculating trapping force using the T-matrix method.Subsequently,it investigates the factors influencing optical tweezers'trapping power in the deep-sea environment,including changes in seawater's refractive index and light wave attenuation.To achieve this,a refractive index model for seawater at 1.5 km north latitude of 18°in the South China Sea is established,and the attenuation coefficient of seawater to light waves is estimated based on prior research data.Furthermore,under consistent experimental parameters,the study employs the T-matrix method to calculate the trapping force of Gaussian optical tweezers in both deep-sea conditions and pure water.This aims to capture spherical microorganisms of varying sizes and refractive indices,assessing the decrease in trapping force of optical tweezers in the deep-sea compared to pure water.Our experimental findings reveal that the shape of the disp
作者
王京
刘博
刘坤香
陈福原
李备
WANG Jing;LIU Bo;LIU Kunxiang;CHEN Fuyuan;LI Bei(State Key Laboratory of Applied Optics,Changchun Institute of Optics,Fine Mechanics and Physics,Chinese Academy of Sciences,Changchun 130033,China;University of Chinese Academy of Sciences,Beijing 100049,China)
出处
《光子学报》
EI
CAS
CSCD
北大核心
2024年第2期160-170,共11页
Acta Photonica Sinica
基金
中国科学院战略性先导科技专项(A类)(No.XDA22020403)。
关键词
光镊
光镊捕获力
T矩阵法
深海环境
深海微生物
海水折射率
Optical tweezers
Optical tweezers capture force
T-matrix
Deep-sea environment
Deepsea microorganisms
Refractive index of seawater
