摘要
为更好的满足市场上不同类型的微生物气溶胶采样器测试需求,进一步指导生物气溶胶采样器的设计、制造、实际应用,提升国产生物气溶胶采样器的研发水平和测试精度,介绍了通过发生粒子模拟微生物气溶胶测试微生物采样器采样效率的荧光法和计数法两种测试装置的组成、测试方法以及数据处理步骤等。同时采用两种测试方法对国产的AGI-30微生物采样器和安德森六级微生物采样器进行测试,得到两款采样器的采样效率结果,以及不同采样条件对采样效率的影响。数据结果表明,AGI-30微生物采样器的采样效率随着发生颗粒粒径的增大而增加,对5μm颗粒的采样效率可达90%以上,且随着采样流量的增大,采样效率逐渐减小。此外,采样时间对采样效率也有明显的影响。安德森六级微生物采样器对8种不同粒径的粒子的采样效率均较高,基本在99%左右,存在随着粒径增大,采样效率增大的趋势。此外,通过两种测试方法的比较,可以明确荧光法和计数法与传统微生物培养法相比较更为简便,且测试过程安全、数据可靠,能够快速得到测试结果。同时荧光法与计数法相比实验过程存在较多步骤,且存在荧光淬灭问题,因此两种方法相比较,可优先选择计数法对生物采样器进行采样效率测试。
To cater to the diverse testing requirements of bioaerosol samplers in the market,and to advance the design,manufacturing,and practical application of these devices,this paper introduces two testing apparatuses—the fluorescence method and the counting method.These methods simulate microbial aerosols to assess the efficiency of microbial samplers.The composition,methodologies,and data processing steps of both testing devices are detailed.Subsequently,these methods were applied to evaluate the sampling efficiency of the domestically produced AGI-30 bioaerosol sampler and the Anderson six-stage microbial sampler under varying conditions.Results show that the AGI-30 sampler's efficiency increases with particle size,exceeding 90%for 5μm particles,and decreases with higher flow rates.Sampling time also significantly impacts efficiency.The Anderson sampler exhibited high efficiency across eight particle sizes,approximately 99%,with a tendency to increase with particle size.The comparative analysis reveals that the fluorescence and counting methods,being more convenient than traditional microbial-culture methods,offer safe,reliable,and rapid results.The counting method,having fewer steps and no fluorescence quenching issues,is preferred for evaluating the efficiency of biological samplers.
作者
马晓丽
邹亚雄
刘巍
王婷
刘文儒
张明
刘伟光
MA Xiaoli;ZOU Yaxiong;LIU Wei;WANG Ting;LIU Wenru;ZHANG Ming;LIU Weiguang(Qingdao Institute of Measurement Technology,Qingdao 266100,China)
出处
《计量科学与技术》
2024年第1期44-52,共9页
Metrology Science and Technology
基金
山东省重点研发计划项目(2019JZZY010419)
国家市场监督管理总局科技计划项目(2020MK120)
山东省市场监督管理局科研项目(2021-12)
山东计量测试学会科技项目(2021KJ38)。
关键词
计量学
微生物气溶胶
采样器
采样效率
测试方法
荧光微球
metrology
bioaerosol
samplers
sampling efficiency
testing methods
fluorescent microspheres
