摘要
目的:探讨睾丸发育相关基因1(TDRG1)在子宫内膜癌患者中的表达及其诊断价值。方法:选取195例子宫内膜癌患者为研究对象(观察组),同期就诊的195例子宫内膜增生患者作为对照组,收集两组子宫内膜组织,采用RT-PCR法检测两组长链非编码RNA-TDRG1(LncRNA-TDRG1)水平,依据观察组LncRNA-TDRG1水平中位数将其分为高表达组和低表达组,分析观察组不同临床特征患者LncRNA-TDRG1表达水平,Logistic回归法分析LncRNA-TDRG1高表达的影响因素。采用ROC曲线分析LncRNA-TDRG1水平对子宫内膜癌的诊断价值。结果:相较于对照组,观察组LncRNA-TDRG1水平更低(P<0.05)。亚组分析结果显示,相较于中高分化、FIGO分期为Ⅰ-Ⅱ、无淋巴结转移患者、无血管侵犯、组织学分级为G1患者,低分化、FIGO分期为Ⅲ-Ⅳ、有淋巴结转移、有血管侵犯,组织学分级为G2-G3患者LncRNA-TDRG1表达水平更高(P<0.01)。低分化、有淋巴结转移、有血管侵犯是LncRNA-TDRG1高表达的危险因素。ROC曲线分析结果显示,LncRNA-TDRG1水平为2.54时,诊断子宫内膜癌的AUC为0.87。结论:子宫内膜癌患者LncRNA-TDRG1呈高表达,且与临床特征有一定联系,在辅助子宫内膜癌诊断中具有一定临床价值。
Objective:To investigate the expression of testicular development related gene 1(TDRG1)in endometrial carcinoma and its diagnostic value.Method:A total of 195 patients with endometrial cancer were selected as the study object(observation group),and 195 patients with endometrial hyperplasia were selected as the control group.Endometrial tissues of the two groups were collected,and the levels of two long chain non-coding RNA-TDRG1(LncRNA-TDRG1)were detected by RT-PCR.The observation group was divided into high expression group and low expression group according to the median level of LncRNA-TDRG1.The expression level of LncRNA-TDRG1 in patients with different clinical characteristics in the observation group was analyzed,and the influencing factors of high expression of LncRNA-TDRG1 were analyzed by Logistic regression method.ROC curve was used to analyze the diagnostic value of LncRNA-TDRG1 in endometrial carcinoma.Results:Compared with control group,the level of LncRNA-TDRG1 in observation group was lower(P<0.05).Subgroup analysis showed that compared with patients with medium-high differentiation,FIGO stageⅠ-Ⅱ,no lymph node metastasis,no vascular invasion,and histological grade G1,patients with low differentiation.FIGO stageⅢ-Ⅳ,lymph node metastasis,and vascular invasion,patients with low differentiation.The expression level of LncRNA-TDRG1 was higher in patients with G2-G3 histological grade(P<0.01).Low differentiation,lymph node metastasis and vascular invasion were risk factors for high expression of LncRNA-TDRG1.ROC curve analysis showed that when the level of LncRNA-TDRG1 was 2.54,the AUC for diagnosis of endometrial cancer was 0.87.Conclusion:LncRNA-TDRG1 is highly expressed in patients with endometrial cancer,which is associated with the severity of the disease.LncRNA-TDRG1 has certain clinical value in the auxiliary diagnosis of endometrial cancer.
作者
王波
王宏
孙艳兰
郭岑川
WANG Bo;WANG Hong;SUN Yan-lan;Guo Cen-chuan(Department of Obstetrics,The Affiliated Huaian NO.1 People’s Hospital of Nanjing Medical University,Huaian 223300,China;Department of Gynaecology,The Affiliated Huaian NO.1 People’s Hospital of Nanjing Medical University,Huaian 223300,China)
出处
《微循环学杂志》
2024年第1期53-57,共5页
Chinese Journal of Microcirculation
基金
淮安市科技局项目(HAB202106)。
关键词
睾丸发育相关基因1
子宫内膜癌
诊断价值
Testis development related gene 1
Endometrial cancer
Diagnosis value