摘要
黄酮类物质是植物次级代谢产物,能够抑制胰脂肪酶活性,具有潜在的调节脂质代谢作用。槲皮素属于黄酮类物质中的黄酮醇类,因此,研究槲皮素及其糖基化衍生物在体内的消化机制,对开发调节脂质代谢功能产品具有重要意义。本试验采用超高效液相色谱-串联质谱(UPLC-MS/MS)的广泛靶向代谢组学技术,分析了板栗皮槲皮素类物质经过模拟人体消化体系后的组成变化。结果表明,板栗皮样品中的槲皮素类物质含量在消化后显著降低(P<0.05),差异代谢物分析显示,下降较为显著的是槲皮素-3-O-β-D-吡喃葡萄糖苷,在模拟胃肠消化后分别降低了94.08%(板栗内皮)和90.88%(板栗外皮)。综上,板栗皮槲皮素类物质在消化过程中稳定性较差,未来可采用纳米颗粒负载等方式对其进行包埋,以提高其生物利用率。
Flavonoids are secondary metabolites of plants,which can inhibit pancreatic lipase and have potential function of regulating lipid metabolism.Quercetin belongs to flavonols in flavonoids.Therefore,it is of great significance to study the digestive mechanism of quercetin and its glycosylated derivatives in vivo to improve the research and development of functional products regulating lipid metabolism.This experiment used a widely targeted metabolomics technique of ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS) to analyze the composition changes of quercetinsubstances in chestnut peel after simulated human digestion system.The results showed that there was a significant difference in the content of quercetinsubstances in chestnut skin samples before and after digestion(P<0.05).Differential metabolite analysis showed that the content of quercetin substances in chestnut skin decreased significantly before and after digestion,with quercetin-3-O-β-D-glucopyranoside decreased by 94.08% chestnut pellicle and 90.88% chestnut shell after simulated gastrointestinal digestion,respectively.In summary,the stability of quercetinsubstances in chestnut peel was poor during digestion.In the future,nanoparticle loading and other methods can be used to embed them to improve their bioavailability.
作者
张文
雷嗣超
钟书言
刘祎妍
杨芳
ZHANG Wen;LEI Sichao;ZHONG Shuyan;LIU Yiyan;YANG Fang(School of Environmental Ecology and Biological Engineering,Wuhan Institute of Technology,Wuhan 430205,China;Key Laboratory of Green Chemical Process(Wuhan Institute of Technology),Wuhan 430205,China;Engineering Research Center of Phosphorus Resources Development and Utilization of Ministry of Education,Wuhan 430205,China;Hubei Key Laboratory of Novel Reactor and Green Chemical Technology,Wuhan 430205,China)
出处
《中国果菜》
2024年第2期18-25,共8页
China Fruit & Vegetable
基金
湖北省大学生创新创业训练计划资助项目(S202310490021)
武汉工程大学校长基金资助项目(XZJJ2023049)。
