摘要
研究旨在建立同时检测畜禽肉中多种驱虫类药物的分析方法,通过优化前处理方法和仪器分析条件,建立了分散固相萃取法-超高效液相色谱-串联质谱法(UPLC-MS/MS)同时测定畜禽肉中23种驱虫类药物残留的分析方法,目标物以乙腈提取,分散固相萃取法(QuEChERS)净化,用乙腈+0.1%甲酸水作为流动相、UPLC-MS/MS确证检测、外标法定量。结果显示:所建立的方法定量限为0.2~1.0μg/kg,回收率为71.4%~107.5%,相对标准偏差为0.9%~8.8%。表明该方法定量限低,稳定性好,适用于畜禽肉中23种驱虫类药物残留的同时检测。
The research aimed to establish an analytical method for the simultaneous detection of multiple insect repellent drugs in livestock and poultry meat.By optimizing the pre-treatment methods and instrument analysis conditions,a dispersion solid-phase extraction ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)method was established for the simultaneous determination of 23 insect repellent drug residues in livestock and poultry meat.The target substance was extracted with acetonitrile,purified with dispersion solid-phase extraction(QuEChERS),and acetonitrile+0.1%formic acid water as the mobile phase UPLC-MS/MS confirmatory detection and external standard quantification.The results show that the established method has a quantitative limit of 0.2-1.0μg/kg,recovery rate 71.4%-107.5%,relative standard deviation 0.9%-8.8%.This method has a low quantitative limit and good stability,and is suitable for the simultaneous detection of 23 insecticidal residues in livestock and poultry meat.
作者
张彤
徐宜宏
付海滨
刘俊
史喜菊
徐蕾蕊
康明芹
张敏
ZHANG Tong;XU Yihong;FU Haibin;LIU Jun;SHI Xiju;XU Leirui;KANG Mingqin;ZHANG Min(College of Engineering,Shenyang Agricultural University,Liaoning Shenyang 110866,China;Technology Center of Shenyang Customs,Liaoning Shenyang 110016,China;Technology Center of Chengdu Customs,Sichuan Chengdu 610041,China;Science and Technology Research Center of China Customs,Beijing 100026,China;Technology Center of Changchun Customs,Jilin Changchun 130062,China)
出处
《饲料工业》
CAS
北大核心
2024年第3期120-126,共7页
Feed Industry
基金
国家重点研发计划项目[2022YFF1100804]
辽宁省自然科学基金项目[2023-MS-213]
四川省科技创新人才项目[2023JDRC0007]
沈阳市科技计划项目[21-108-9-37]。
关键词
驱虫药
超高效液相色谱-串联质谱
畜禽肉
药物残留
分散固相萃取法
vermifuge
ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)
livestock and poultry meat
residual
QuEChERS
