摘要
以赤芝(Ganoderma lucidum)、人参(Panax ginseng)根、天山雪莲(Saussurea involucrata)为原料,采用双相连续油提技术提取,提取物复配氢化聚异丁烯和牡丹籽油制得牡丹籽油复合提取物。通过2D成纤维细胞模型,探索牡丹籽油复合提取物的最大安全剂量,并以该最大安全剂量作为上限,采用3D全层皮肤光老化损伤模型,评估牡丹籽油复合提取物对皮肤光老化的修护作用。结果显示,牡丹籽油复合提取物对成纤维细胞的最大安全剂量为0.25%(体积分数);体积分数0.05%~0.20%牡丹籽油复合提取物可明显改善表皮细胞形态、降低UV对表皮层厚度的破坏以及真皮层成纤维细胞密度等,且能显著提升Ⅳ型胶原蛋白(Collagen IV)表达(P<0.01),通过减少产生CPD细胞的数量来降低DNA损伤(P<0.01),也说明其具有良好的修护光老化功效。
Ganoderma lucidum,Panax ginseng and Saussurea involucrata(Tianshan snow lotus)were extracted by biphasic continuous oil extraction technology as raw materials,and the plant complex extract was compounded with hydrogenated polyisobutylene and peony seed oil with a certain mass ratio to obtain the compound peony seed oil extract.Through the 2D fibroblast model,the maximum safe dose of the compound peony seed oil extract was explored,and this maximum safe dose was used as the upper limit to evaluate the repairing effect on skin photoaging by using a 3D skin model of full layer(FulKutis®)skin photoaging damage model.The results showed that the maximum safe dose of the compound peony seed oil extract on fibroblasts was 0.25%(in volume fraction);0.05%~0.20%(in volume fraction)of this compound extract could clearly improve the morphology of epidermal cells,reduce the UV damage to the thickness of the epidermal layer as well as the density of fibroblasts in the dermal layer,etc.,and it could significantly enhance the expression of type Collagen IV expression(P<0.01)and reduced DNA damage by decreasing the number of CPD-producing cells(P<0.01),which also indicated its good efficacy in repairing photoaging.
作者
亓玉锋
郭晓丹
张晔翔
韦笑余
张卫红
任晗堃
QI Yufeng;GUO Xiaodan;ZHANG Huaxiang;WEI Xiaoyu;ZHANG Weihong;REN Hankun(Shandong Huawutang Biotechnology Co.,Ltd.,Jinan 250000,Shandong,China;Beijing Academy of TCM Beauty Supplemengts Co.,Ltd.,Beijing 102401,China)
出处
《香料香精化妆品》
CAS
2024年第1期84-91,共8页
Flavour Fragrance Cosmetics
关键词
牡丹籽油复合提取物
3D全层皮肤模型
光老化
修护
the compound peony seed oil extract
3D skin model of full layer
photoaging repair
