摘要
【目的】阐明MYB基因家族成员是否参与大豆叶柄角(leaf petioles angle,LPA)变化的调控过程,为大豆理想株型育种研究奠定基础。【方法】以郑196为材料,利用大豆叶枕响应叶片向光运动转录组挖掘差异表达大豆MYB基因(GmMYB);利用ProtComp 9.0进行亚细胞定位预测;以拟南芥MYB蛋白序列为参考,采用MEGA v5.0软件进行系统进化分析;分别利用在线程序MEME和PlantPAN3.0分析差异表达MYB基因保守基序(motif)和启动子序列的顺式作用元件;以NCBI数据库中大豆转录组数据及RNA-seq数据为基础,采用TBtools进行表达模式分析,并在此基础上进行候选基因的精准定量检测。【结果】①鉴定出13个差异表达GmMYB,其中上调表达9个,下调表达4个;亚细胞定位结果表明,11个MYB蛋白位于细胞核,2个位于细胞外基质。②基于MYB蛋白序列构建系统进化树,可将13个差异表达GmMYB分为6组。③对13个差异表达GmMYB基因结构进行分析,结果共鉴定到10个基序,其中基序1和基序2是MYB保守结构域的一部分。④在启动子区域鉴定获得脱落酸响应元件、参与干旱响应元件、响应光照的顺式作用元件、分生组织表达和生长素响应等多个响应逆境胁迫及光照的顺式作用元件。⑤表达模式分析结果表明,13个差异表达GmMYB基因在大豆不同组织器官中存在表达差异,其中Glyma.01G190100和Glyma.08G042100在叶枕处表达。⑥对Glyma.01G190100和Glyma.08G042100进行精准定量检测,结果显示其相对表达量在叶片向光运动前后变化剧烈,可作为大豆叶柄角调控候选基因用于进一步研究。【结论】鉴定出13个与大豆叶柄角调控相关的MYB基因,其中Glyma.01G190100和Glyma.08G042100可作为大豆叶柄角调控候选基因用于大豆理想株型育种研究。
【Objective】This study clarified whether members of the MYB gene family are involved in the regulation of soybean leaf petiole angle(LPA)changes to lay the foundation for breeding ideal plant types of soybean.【Method】Differentially expressed GmMYB genes of‘Zheng196’were identified from the transcriptome of pulvinus responses to light-directed leaf movement.Subcellular location was predicted using ProtComp 9.0.Phylogenetic analysis was carried out using MEGA v5.0 software with Arabidopsis MYB protein sequences as reference.Conserved motifs and cis-acting elements of differentially expressed MYB genes were analyzed using online programs of MEME and PlantPAN3.0,respectively.Based on transcriptome data in the NCBI database and RNA-seq data,expression pattern analysis was performed using TBtools,and the candidate genes were precisely and quantitatively detected.【Result】①A total of 13 di-fferentially expressed GmMYBs were identified,of which 9 were up-regulated and 4 were down-regulated.The subcellular localization indicated that 11 MYB proteins were located in the nucleus and 2 were located extracellular matrix.②A phylogenetic tree was constructed based on MYB protein sequences,and the 13 differentially expressed GmMYBs could be classified into six groups.③Structural analysis of the 13 differentially expressed GmMYB genes resulted in the identification of 10 motifs,of which motif 1 and motif 2 were parts of the conserved structural domain of MYB.④Several cis-acting elements were identified in the promoter region in response to ABA,drought,light,meristem expression and auxin.⑤Expression analysis showed that the 13 GmMYB genes were differentially expressed in different tissues and organs of soybean,among which Glyma.01G190100 and Glyma.08G042100 were expressed at the pulvinus.⑥The quasi-quantitative assays of Glyma.01G190100 and Glyma.08G042100 also showed that these two genes varied drastically before and after light-directed leaf movement,and they were identified as candidate genes for furth
作者
常玮
王娟
郑清岭
张彦威
陈吉宝
CHANG Wei;WANG Juan;ZHENG Qingling;ZHANG Yanwei;CHEN Jibao(College of Life Science and Agricultural Engineering,Nanyang Normal University,Nanyang,Henan 473000,China;Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan,Shandong 250100,China)
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2024年第2期60-69,共10页
Journal of Northwest A&F University(Natural Science Edition)
基金
河南省科技攻关计划(农业领域)项目(192102110125)
山东省自然科学基金(ZR2020MC101)
南阳师范学院青年项目(2023QN009)。
关键词
大豆
叶柄角
叶枕
转录组
MYB转录因子
soybean
petiole angle
pulvinus
transcriptome
MYB transcriptional factor
