摘要
采用基于液质联用的非靶向代谢组学技术结合化学计量学数据自动解析软件AntDAS-LCHRMS分析了4种不同干燥处理(冻干、热泵烘干、电热烘干、晒干)杜仲叶样本中的化合物。杜仲叶样本数据由超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UHPLC-Q-Exactive Orbitrap HRMS)分别在正、负离子模式下进行采集,经AntDAS-LCHRMS软件解析,共鉴定出71种差异性化合物,经标准品验证确定40种化合物,包括环烯醚萜类、有机酸类、黄酮类、氨基酸类、核苷类、维生素类等9类物质。其中,正、负离子模式下均可识别并验证的化合物有车叶草苷、绿原酸、芦丁、异槲皮苷、车叶草苷酸、京尼平苷等25种化合物。层次聚类分析(HCA)及主成分分析(PCA)结果均显示,相同处理的杜仲叶样本各自聚成一类,不同处理的杜仲叶样本可明显区分。热图分析进一步揭示了不同干燥处理杜仲叶样本中差异性化合物的含量变化。晒干处理样本中苯丙氨酸及色氨酸等氨基酸类物质的水平较高;冻干及热泵烘干处理样本中有机酸类、环烯醚萜类、糖类等含量较高;电热烘干样本中核苷类、黄酮类物质的含量较高;黄酮类物质在冻干、热泵烘干及晒干样本中差异较小。研究结果为不同干燥处理杜仲叶的成分分析、品质评价及其开发应用提供了科学依据,也可为其他复杂药用植物体系的化学成分分析提供参考。
A strategy that ultrahigh performance liquid chromatography-quadrupole-exactive orbitrap high resolution mass spectrometry(UHPLC-Q-Exactive orbitrap HRMS)based untargeted metabolomics was developed in combined with chemometric automatic data analysis software(AntDASLCHRMS)to investigate the metabolites from four types of E.ulmoides Oliv.leaves processed by different drying treatments(i.e.,freeze drying,heat pump drying,oven drying,sun drying).The data of samples was acquired by UHPLC-Q-Exactive orbitrap HRMS under both positive ionization mode and negative ionization mode,and directly imported into AntDAS-LCHRMS for automatic data analysis.The results indicated that about 71 differential metabolites were identified based on the above-mentioned data analysis workflow,and 40 compounds were further confirmed by standards,including iridoids,organic acids,flavonoids,amino acids,nucleosides,vitamins,sugars,lignans,and coumarins.Among of which 25 metabolites were identified and confirmed by standards under both positive and negative mode,including asperuloside,chlorogenic acid,rutin,isoquercitrin,asperulosidic acid and geniposide,etc.Finally,hierarchical clustering analysis(HCA),principal component analysis(PCA),and heatmap analysis were used for discriminating samples from various drying treatments.The results showed that Eucommiae Folium obtained from different drying treatments could be clearly separated,and compounds of them were significantly different.Heatmap analysis further explicated the compound variations in E.ulmoides Oliv.leaves from different drying groups.Samples of sun drying had higher levels of amino acids such as phenylalanine and tryptophan.Samples of freeze drying and heat pump drying had higher levels in organic acids,iridoids,and sugars.Samples of oven drying had higher levels of nucleotides and flavonoids.The content of flavonoids differed little among freeze drying,heat pump drying,and sun drying.The results could be helpful to offer a scientific guidance for the quality evaluation and applicati
作者
李淑芳
王会锋
郝学飞
胡永建
李圆圆
马风莲
冯书惠
杨亚琴
于永杰
LI Shu-fang;WANG Hui-feng;HAO Xue-fei;HU Yong-jian;LI Yuan-yuan;MA Feng-lian;FENG Shu-hui;YANG Ya-qin;YU Yong-jie(Institute of Quality and Safety for Agro-products,Henan Academy of Agricultural Science,Zhengzhou 450002,China;College of Pharmacy,Ningxia Medical University,Yinchuan 750004,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2024年第2期213-225,共13页
Journal of Instrumental Analysis
基金
国家自然科学基金资助项目(21868028,22378214)
河南省重点研发与推广专项(科技攻关)项目(232102110152)
宁夏回族自治区优秀青年基金(2023AAC05038)
宁夏回族自治区重点研发计划项目(2022BEG03170)
河南省农业科学院自主创新专项基金(2023ZC075)。
