摘要
目的观察大半夏汤提取物对食管癌EC9706细胞恶性生物学行为及JAK1/STAT3通路的影响,以期为食管癌的防治提供参考。方法人食管癌EC9706细胞分别以半夏汤提取物(200、100、50、25、12.5μg/mL)、STAT3信号通路抑制剂(Stattic,4、3.5、3、2.5、2μmol/L)处理,MTT法检测细胞抑制率,拟合细胞抑制率-浓度曲线计算50%抑制浓度(IC_(50))。人食管癌EC9706细胞分为空白对照组、大半夏汤组、Stattic组与大半夏汤+Stattic组。大半夏汤组、Stattic组分别以IC_(50)浓度的大半夏汤提取物及Stattic干预,大半夏汤+Stattic组予以大半夏汤提取物及Stattic联合干预,空白对照组不做特殊处理。MTT法检测细胞增殖能力;划痕实验检测细胞迁移能力;Transwell实验检测各组GES-1细胞侵袭能力;qRT-PCR法及Westernblotting法检测JAK1/STAT3信号通路相关mRNA及蛋白表达情况。结果随着大半夏汤提取物、Stattic干预浓度的增加,EC9706细胞的抑制率增加(P<0.05);拟合细胞抑制率-浓度曲线,结果显示大半夏汤提取物与Stattic的曲线拟合优度结果均为优(R^(2)=0.957、0.961),其IC_(50)值分别为98.50μg/mL、3.82μmol/L。与空白对照组比较,大半夏汤组、Stattic组、大半夏汤+Stattic组的OD值、迁移率和侵袭数量更低(P<0.05);与Stattic组及大半夏汤组比较,大半夏汤+Stattic组的OD值、迁移率和侵袭数量更低(P<0.05)。与空白对照组比较,大半夏汤组、Stattic组、大半夏汤+Stattic组的JAK1mRNA、STAT3mRNA表达量更低(P<0.05);与Stattic组及大半夏汤组比较,大半夏汤+Stattic组的JAK1mRNA、STAT3mRNA表达量更低(P<0.05)。与空白对照组比较,大半夏汤组与大半夏汤+Stattic组p-JAK1、p-STAT3蛋白表达量更低(P<0.05);与大半夏汤组比较,大半夏汤+Stattic组p-JAK1蛋白表达量更低(P<0.05);与Stattic组及大半夏汤组比较,大半夏汤+Stattic组的p-STAT3蛋白表达量更低(P<0.05)。结论大半夏汤提取物能抑制食管癌EC9706细�
Objective:To analyze the effects of extracts of Dabanxia Decoction extract on malignant biological behavior and JAK1/STAT3 pathway of esophageal cancer EC9706 cells,in order to provide reference for the prevention and treatment of esophageal cancer.Methods:Human esophageal cancer EC9706 cells were treated with Dabanxia Decoction extract(200,100,50,25,12.5μg/mL)and STAT3 signaling pathway inhibitor(Stattic,4,3.5,3,2.5,2μmol/L),and the cell inhibition rate was detected by MTT method.The 50%inhibitory concentration(IC_(50))was calculated by fitting the cell inhibition rate-concentration curve.Human esophageal cancer EC9706 cells were divided into blank control group,Dabanxia Decoction group,Stattic group and Dabanxia Decoction+Stattic group.Dabanxia Decoction group and Stattic group were treated with IC_(50)concentration of Dabanxia Decoc-tion extract and Stattic respectively.Dabanxia Decoction+Stattic group was treated with Dabanxia Decoction ex-tract and Stattic combined intervention.No special treatment was done in the blank control group.Cell prolifera-tion was detected by MTT assay.Cell migration ability was detected by scratch test.The invasion ability of GES-1 cells in each group was detected by Transwell assay.The mRNA and protein expression of JAK1/STAT3 signaling pathway were detected by qRT-PCR and Western blotting.Results:The inhibitory rate of EC9706 cells increased with the increase of Stattic and Dabanxia Decoction extract(P<0.05).The results showed that the curves of Stattic and the Dabanxia Decoction extract were both excellent(R^(2)=0.957,0.961),and their IC_(50)values were 98.50μg/mL and 3.82μmol/L,respectively.Compared with blank control group,the OD value,mobility and invasion number of Dabanxia Decoction group,Stattic group and Dabanxia Decoction+Stattic group were lower(P<0.05).Compared with Stattic group and Dabanxia Decoction group,the OD value,mobility and invasion number in Dabanxia Decoction+Stattic group were lower(P<0.05).Compared with blank control group,the expressions of JAKI mRN
作者
马燕凌
叶子豪
晏菲
魏武杰
李黎
刘莉
孙建海
Ma Yanling;Ye Zihao;Yan Fei;Wei Wujie;Li Li;Liu Li;Sun Jianhai(Hubei No.3 People's Hospital of Jianghan University,Hubei,Wuhan 430000,China)
出处
《中国中医急症》
2024年第2期204-208,共5页
Journal of Emergency in Traditional Chinese Medicine
基金
湖北省中医药管理局中医药科研项目(ZY2023F031)。
