摘要
茶藨子葡萄座腔菌Botryosphaeria dothidea是一种世界性分布真菌,能引起山核桃干腐病,导致严重经济损失。为研究该病害的致病机理及筛选致病基因,通过各类预测与分析工具,对13130条蛋白序列的山核桃Carya cathayensis茶藨子葡萄座腔菌BDLA16-7全基因组进行了分泌蛋白预测及其功能分析。结果表明,在BDLA16-7全基因组的编码蛋白序列中,有898条蛋白序列符合典型分泌蛋白的特征,占总蛋白数的6.84%;分泌蛋白组的氨基酸组成呈现以下特征:非极性、疏水性氨基酸的含量明显高于极性、亲水性氨基酸,分别占43.6%和27.7%;蛋白质长度主要分布在100至600个氨基酸之间;分泌蛋白序列中通常以19个氨基酸构成信号肽,丙氨酸(A)是最常见的非极性氨基酸,而带电侧链的天冬氨酸(D)和谷氨酸(E)的出现频率较低;信号肽的切割位点-3和-1位置上的氨基酸相对保守,典型的氨基酸组合为A-S-A,符合A-X-A型的特征。此外,分泌蛋白组中包含600个序列可以完整注释其功能,其中229个分泌蛋白主要涉及碳水化合物的运输和代谢。在BDLA16-7分泌蛋白组中,共存在271个潜在的效应蛋白,其中14个与其他病原菌致病相关的效应蛋白具有较高的相似性。此外,BDLA16-7分泌蛋白组中包含293个碳水化合物活性酶,以糖苷水解酶家族成员为最多。明确了茶藨子葡萄座腔菌BDLA16-7分泌蛋白的长度分布特征,功能分类、信号肽切割位点的氨基酸组成、切割位点的类型和CAZymes数目,以及CAZymes中纤维素酶、果胶酶、木聚糖酶的亚家族组成,并筛选到14个与致病性紧密联系的潜在效应蛋白。
The full genome of Botryosphaeria dothidea strain BDLA16-7,a pathogenic fungus from an infected Carya cathayensis tree,comprising 13130 protein sequences,which were analyzed to predict and functionally characterize secretory proteins by various prediction and analysis tools.The results indicated that in the encoded protein sequences of the BDLA16-7 whole genome,there were 898 sequences typical characteristics of secretory proteins,accounting for 6.84%of the total ones.The amino acid composition of the secretory protein group showed the following characteristics:the content of non-polar and hydrophobic amino acids was significantly higher than that of polar and hydrophilic amino acids,accounting for 43.6%and 27.7%,respectively.The length of the protein is mainly distributed between 100 and 600 amino acids.Alanine(A)is the most common non-polar amino acid,while aspartic acid(D)and glutamic acid(E)with charged side chains appear less frequently.The amino acids at the cleavage sites-3 and-1 of the signal peptide are relatively conserved,and the typical amino acid combination is A-S-A,which conforms to the characteristics of A-X-A type.Within the secretory protein group,600 sequences could be fully annotated for their functions,among them,229 involved in the transport and metabolism of carbohydrates.Among the BDLA16-7 secretory proteins,there were a total of 271 potential effector proteins,with 14 of them showing high similarity to effectors associated with other pathogenic organisms.BDLA16-7 secretory protein group contains 293 CAZymes,dominated by GHs family members.Analysis were made on the length distribution characteristics,functional classification,amino acid composition of signal peptide cleavage site,type of cleavage site and number of CAZymes of secretory proteome of B.dothidea,as well as on the subfamily composition of cellulase,pectinase and xylanase in CAZymes.14 potential effectors closely related to pathogenicity were screened.
作者
吴怡豪
诸葛君昊
张立华
周乐
杜盛楠
杜世平
苏秀
WU Yihao;ZHUGE Junhao;ZHANG Lihua;ZHOU Le;DU Shengnan;DU Shiping;SU Xiu(College of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou 311300,China;Taishun Natural Resources and Planning Bureau of Zhejiang,Wenzhou 325500,China;Linhai Hetou Township Agricultural Service of Zhejiang,Linhai 317034,China)
出处
《浙江林业科技》
2024年第1期8-18,共11页
Journal of Zhejiang Forestry Science and Technology
基金
浙江省重点研发项目(2019C02024)。
关键词
茶藨子葡萄座腔菌
分泌蛋白
效应蛋白
功能
Botryosphaeria dothidea
secretory proteome
effector proteins
function
