摘要
以脂肪酶(lipase,CALB)为模型酶,利用功能性纳米材料四氧化三铁纳米粒子与金属有机骨架(metal-organic framework,MOF)材料ZIF-8相杂化的同时,将CALB包埋在杂化材料中,制备杂化纳米生物催化剂CALB@Fe_(3)O_(4)@ZIF-8,研究了温度、机械搅拌转速、光照条件以及Fe_(3)O_(4)添加量对CALB@Fe_(3)O_(4)@ZIF-8活性的影响,获得制备CALB@Fe_(3)O_(4)@ZIF-8的最佳条件为:温度20℃、转速400 r/min、光照波长380 nm、Fe_(3)O_(4)质量5 mg。在此条件下CALB@Fe_(3)O_(4)@ZIF-8的最大酶活回收率82%,比优化前提高了16%。随着具有光热效应的Fe_(3)O_(4)纳米粒子的加入,CALB@Fe_(3)O_(4)@ZIF-8表现出较强的光热效应,光照(光强和光照波长)显著提高酶与底物的亲和力和反应速率,使CALB@Fe_(3)O_(4)@ZIF-8表现出更高的酶活力。该研究为开发新型功能性纳米杂化MOF固定化酶生物催化剂提供参考。
Lipase(CALB)was used as the model enzyme.When functional nanomaterial ferric oxide nanoparticles were hybridized with metal-organic framework(MOF)material ZIF-8,CALB was embedded in the hybrid material at the same time to prepare hybrid nanobiocatalysts CALB@Fe_(3)O_(4)@ZIF-8.The effects of temperature,mechanical stirring speed,illumination and the amount of Fe_(3)O_(4)on the activity of CALB@Fe_(3)O_(4)@ZIF-8 catalyst were investigated.The optimum conditions for preparing CALB@Fe_(3)O_(4)@ZIF-8 were as follows:temperature 20℃,rotation speed 400 r/min,illumination wavelength 380 nm and Fe_(3)O_(4)mass 5 mg.Under these conditions,the maximum recovery rate of CALB@Fe_(3)O_(4)@ZIF-8 was 82%,which was 16%higher than before optimization.At the same time,with the addition of Fe_(3)O_(4)nanoparticles with photothermal effect,CALB@Fe_(3)O_(4)@ZIF-8 showed strong photothermal effect,light intensity and wavelength could significantly improve the affinity and reaction rate of enzyme and substrate,and CALB@Fe_(3)O_(4)@ZIF-8 showed higher apparent enzyme activity.This study provides a useful reference for the development of novel functional nanohybrid MOF immo-bilized enzyme biocatalysts.
作者
杜英杰
贾晓彤
罗秀艳
崔建东
贾士儒
龚栋
DU Yingjie;JIA Xiaotong;LUO Xiuyan;CUI Jiandong;JIA Shiru;GONG Dong(College of Biotechnology,Tianjin University of Science&Technology,Tianjin 300457,China;Hubei Hanjiang Great Health Industry Co.,Ltd.,Shiyan 442000,China;Tianjin Ubasio Biotechnology Group Co.,Ltd.,Tianjin 300457,China)
出处
《天津科技大学学报》
CAS
2024年第1期8-14,49,共8页
Journal of Tianjin University of Science & Technology
基金
国家自然科学基金青年项目(22108206)
中国博士后科研基金面上项目(2022M722387)。
关键词
生物催化
金属有机骨架材料
纳米材料
固定化酶
光热效应
biocatalysis
metal organic framework materials
nanomaterial
immobilized enzyme
photothermal effect
