摘要
目的:探讨p16/Ki-67双染检测、HPVE6/E7mRNA检测在宫颈病变诊断中的价值。方法:选择2021年4月至2023年2月我院收治的605例高危型HPV阳性患者为研究对象,所有患者入院后均进行阴道镜检查与病理活检,取患者细胞学样本分别进行p16/Ki-67双染检测、HPVE6/E7mRNA检测。比较两种方法单独检查与联合检测对宫颈病变阳性检出率的差异;比较三种检查方法对宫颈病变诊断效能的差异。结果:联合检测检出CIN1~3阳性率均高于p16/Ki-67双标记、HPVE6/E7mRNA检测(P<0.05);三种检测方式的浸润癌阳性检出率比较无明显差异(P>0.05)。Kappa检验分析结果显示,p16/Ki-67双标记与HPVE6/E7mRNA检查结果与病理诊断结果具有较好的一致性(Kappa值=0.719,P<0.05);联合检测结果与病理诊断结果具有高度的一致性(Kappa值=0.894,P<0.05)。联合检测对宫颈病变的诊断灵敏度、特异性与准确率高于p16/Ki-67双标记与HPVE6/E7mRNA检查(P<0.05)。结论:p16Ki-67双染与HPVE6/E7mRNA联合检测可准确检出宫颈病变程度,对宫颈病变疾病类型的鉴别可提供充足可靠的数据支持,联合检查的诊断效能较好,对患者后续相关治疗开展有积极指导意义。
Objective:To investigate the value of p16/Ki-67 double staining and HPVE6/E7mRNA detection in the diagnosis of cervical lesions.Methods:605 high-risk HPV-positive patients admitted to our hospital from April 2021 to February 2023 were selected as the study objects.Colposcopy and pathological biopsy were performed on all patients after admission,and cytological samples were collected for p16/Ki-67 double staining and HPVE6/E7mRNA detection,respectively.The positive detection rates of cervical lesions were compared between the two methods.To compare the efficacy of three methods in the diagnosis of cervical lesions.Results:The positive rates of CIN1-3 detected by combined assay were higher than those detected by p16/Ki-67 double labeling and HPVE6/E7mRNA(P<0.05).There was no significant difference in the positive detection rate of invasive carcinoma among the three methods(P>0.05).The results of Kappa test showed that the results of p16/Ki-67 double labeling and HPVE6/E7mRNA were consistent with the pathological diagnosis results(Kappa value=0.719,P<0.05).The results of combined detection were highly consistent with those of pathological diagnosis(Kappa=0.894,P<0.05).The diagnostic sensitivity,specificity and accuracy of combined detection for cervical lesions were higher than p16/Ki-67 double labeling and HPVE6/E7mRNA detection(P<0.05).Conclusion:The combined detection of p16Ki-67 double staining and HPVE6/E7mRNA can accurately detect the degree of cervical lesions,and provide sufficient and reliable data support for the identification of cervical lesions and disease types.The combined detection has good diagnostic efficacy,and has positive guiding significance for the follow-up treatment of patients.
作者
彭海兰
梁土玉
黄之文
李德峰
Peng Hailan(Gaozhou People’s Hospital,Maoming Guangdong 525200)
出处
《黑龙江医药》
CAS
2024年第1期41-44,共4页
Heilongjiang Medicine journal
基金
茂名市科技计划项目(项目编号:210423234557325)。
