摘要
目的:分析孤独症谱系障碍儿童的肠道菌群特点及其对患儿行为的影响。方法:选取2022年6—9月莆田学院附属医院医疗集团收治的30例孤独症谱系障碍患儿,将其纳入孤独症谱系障碍组,同期抽选20例年龄和性别与之匹配的健康儿童纳入对照组。收集粪便标本,采用基因组学病原微生物分析检测技术测定肠道菌群分布情况,采用信息生物学方法筛选出自闭症相关病原微生物。对比两组肠道菌群丰富度指数、各菌群相对丰度、自闭症治疗评估量表(autism treatment evaluation checklist,ATEC)评分,分析肠道菌群特点与行为症状的相关性。结果:两组Sobs、Chao、基于丰度的覆盖估计值(abundance-based coverage estimator,Ace)比较,差异无统计学意义(P>0.05);在操作分类单元(operational taxonomic units,OTU)水平,孤独症谱系障碍组香农(Shannon)指数和香农威纳(Shannoneven)指数低于对照组,差异有统计学意义(P<0.05)。孤独症谱系障碍组Solobacterium属、普雷沃菌属、杆菌属的相对丰度低于对照组,小球菌属、丛毛单胞菌属、伯克氏菌属、霍尔德曼菌属、另枝菌属的相对丰度高于对照组,差异有统计学意义(P<0.05)。孤独症谱系障碍组ATEC评分高于对照组,差异有统计学意义(P<0.05)。孤独症谱系障碍患儿Shannon指数、Shannoneven指数、各菌相对丰度与行为症状具有相关性(P<0.001)。结论:孤独症谱系障碍儿童肠道菌群结构与其行为症状密切相关。
Objective:To analyze the characteristics of intestinal flora in children with autism spectrum disorder and its influence on their behavior.Method:Thirty children with autism spectrum disorder who admitted to Affiliated Hospital of Putian University Medical Group from June to September 2022 were included in the autism spectrum disorder group,and 20 healthy children matching age and sex were selected to be included in the control group during the same period.Fecal samples were collected,and the distribution of intestinal flora was determined by genomic pathogenic microorganism analysis and detection technology,and the autism-related pathogenic microorganisms were screened by information biology method.The intestinal flora richness index,the relative abundance of each flora and the autism treatment evaluation checklist(ATEC)score of two groups were compared,the correlation between intestinal flora characteristics and behavioral symptoms were analyzed.Result:There were no statistically significant differences in Sobs,Chao and abundance-based coverage estimator(Ace)between two groups(P>0.05);at the operational taxonomic units(OTU)level,the Shannon index and Shannoneven index of the autism spectrum disorder group were lower than those of the control group,and the differences were statistically significant(P<0.05).The relative abundance of Solobacterium,Prevotella and Bacillus in the autism spectrum disorder group were lower than those in the control group,and the relative abundance of Micrococcus,Comamonas,Burkholderia,Haldemania and Alistipes were higher than those in the control group,and the differences were statistically significant(P<0.05).The ATEC scores of the autism spectrum disorder group were higher than those of the control group,and the differences were statistically significant(P<0.05).The Shannon index,Shannoneven index and relative abundance of each bacteria in children with autism spectrum disorder were correlated with behavioral symptoms(P<0.001).Conclusion:The intestinal flora structure of children wi
作者
韩俊林
林玉梅
林堃
韩新容
吴星星
周少君
林一珍
HAN Junlin;LIN Yumei;LIN Kun;HAN Xinrong;WU Xingxing;ZHOU Shaojun;LIN Yizhen(Affiliated Hospital of Putian University Medical Group,Putian 351100,China;不详)
出处
《中外医学研究》
2023年第34期63-67,共5页
CHINESE AND FOREIGN MEDICAL RESEARCH
基金
莆田学院科技项目(2022073)。
关键词
孤独症谱系障碍
肠道菌群特点
行为
影响因素
Autism spectrum disorder
Characteristics of intestinal flora
Behavior
Influencing factor
