摘要
目的机械清除对种植体周炎的治疗效果预后差,需要辅助以局部抗菌药物的应用。肉桂醛(cinnamaldehyde,CA)具有抑菌作用,但其在种植体周炎中的应用仍不清楚。本研究拟探究CA对种植体周炎的主要致病菌牙龈卟啉单胞菌(Porphyromonas gingivalis,P.gingivalis)的抑制作用,并进一步通过等离子及碱热处理在钛表面负载CA,为其在体内应用提供可能。方法通过倍比稀释法明确CA对P.gingivalis的最低抑菌浓度及最低抗菌浓度,及其对P.gingivalis产生的牙龈素活性的影响;随后通过碱热联合等离子体处理钛表面,检测其亲水性以及对CA的负载能力及缓释能力;最后将P.gingivalis培养于不同处理的钛表面,检测CA的抗菌性能。结果CA对P.gingivalis的最低抑菌浓度和最低杀菌浓度分别为21.12μg/mL和42.24μg/mL,1/2/及1/4最低抑菌浓度的CA对P.gingivalis产生的牙龈素具有显著的抑制作用。碱热处理和等离子-碱热复合处理能显著提升钛片的亲水性,均可负载CA,对P.gingivalis的黏附和生长具有抑制作用,等离子-碱热复合处理的负载量及抗菌效果优于碱热处理组。结论CA可以抑制P.gingivalis的生长及毒性,采用等离子-碱热复合处理可实现CA的负载,增强钛片的抗菌作用,有利于种植体周炎的预防和治疗。
Objective Mechanical debridement exhibits a poor prognosis in the treatment of peri-implantitis,necessitating the adjunctive use of local antimicrobial agents.Cinnamaldehyde(CA)possesses antimicrobial properties,yet its application in peri-implantitis remains unclear.This study aimed to investigate the inhibitory effects of cinnamaldehyde on the primary pathogen,Porphyromonas gingivalis(P.gingivalis),in peri-implantitis.Furthermore,the study explored the loading of cinnamaldehyde onto titanium surfaces through plasma plus alkali and heat treatment for potential in vivo applications.Methods The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of cinnamaldehyde on P.gingivalis were determined by serial dilution.Its impact on gingipain activity of P.gingivalis was evaluated.Subsequently,titanium surfaces were subjected to alkali and heat treatment combined with or without plasma treatment to assess its hydrophilicity,drug-loading capacity,and sustained release of cinnamaldehyde.P.gingivalis cultures on differently treated titanium surfaces were examined for antimicrobial properties.Results The MIC and MBC of cinnamaldehyde on P.gingivalis were found to be 21.12μg/mL and 42.24μg/mL,respectively.Cinnamaldehyde at 1/2 and 1/4 MIC significantly inhibited the gingipain produced by P.gingivalis.Alkali and heat treatment with or without plasma treatment significantly enhanced the hydrophilicity of titanium surfaces and facilitated cinnamaldehyde loading,exerting inhibitory effects on the adhesion and growth of P.gingivalis.The composite treatment outperformed the alkali and heat treatment alone in terms of loading capacity and antimicrobial efficacy on P.gingivalis.Conclusion Cinnamaldehyde effectively inhibits the growth and virulence of P.gingivalis.Plasma-alkali composite treatment enables successful loading of cinnamaldehyde,augmenting the antibacterial properties of titanium surfaces and offering promise for periimplantitis prevention and therapy.
作者
欧艳晶
陆洁
陈江
Ou Yanjing;Lu Jie;Chen Jiang(Fujian Key Laboratory of Oral Diseases&Fujian Provincial Engineering Research Center of Oral Biomaterial&Stomatology Key Lab of Fujian College and University,School and Hospital of Stomatology,Fujian Medical University,Fuzhou 350004,Fujian,China;School of Stomatology,Fujian Medical University,Craniomaxillofacial Implant Research Center,Fujian Medical University,School and Hospital of Stomatology,Fujian Medical University,Fuzhou 350004,Fujian,China)
出处
《中国口腔种植学杂志》
2023年第6期410-416,共7页
Chinese Journal of Oral Implantology
关键词
种植体周炎
肉桂醛
碱热
等离子体
牙龈卟啉单胞菌
Peri-implantitis
Cinnamaldehyde
Alkali and heat treatment
Plasma
Porphyromonas gingivalis
