摘要
目的评价小檗碱对吗啡诱导BV2小胶质细胞活化的影响。方法 BV2小胶质细胞采用随机数字表法分为3组(n=12):对照组(C组)、吗啡组(Mor组)和吗啡+小檗碱组(Mor+BBR组)。Mor组使用终浓度为200 μmol/L吗啡处理24 h, C组加入等量PBS处理24 h。Mor+BBR组使用终浓度为20 μmol/L小檗碱处理2 h后再使用终浓度为200 μmol/L吗啡处理24 h。采用CCK-8法测定BV2小胶质细胞活力, ELISA法测定上清液IL-1β、TNF-α和IL-10浓度, Western blot法检测小胶质细胞CD86和NF-κB的表达。结果与C组比较, Mor组细胞活力、上清液IL-1β和TNF-α浓度升高, IL-10浓度降低, 小胶质细胞CD86和NF-κB表达上调(P<0.05);与Mor组比较, Mor+BBR组细胞活力、上清液IL-1β和TNF-α浓度降低, IL-10浓度升高, 小胶质细胞CD86和NF-κB表达下调(P<0.05)。结论小檗碱可抑制吗啡诱导BV2小胶质细胞的活化。
Objective To evaluate the effect of berberine(BBR)on morphine-induced activation of BV2 microglial cells.Methods The BV2 microglial cells were divided into 3 groups(n=12 each)using a random number table method:control group(C group),morphine group(Mor group)and morphine+BBR group(Mor+BBR group).The Mor group was treated for 24 h with a final concentration of 200μmol/L morphine,while C group was treated for 24 h with an equal volume of PBS buffer.Mor+BBR group was first treated for 2 h with a final concentration of 20μmol/L berberine,followed by treatment with a final concentration of 200μmol/L morphine for another 24 h.The viability of BV2 microglial cells was determined using the CCK-8 assay,the concentrations of interleukin^(-1)beta(IL-1β),tumor necrosis factor-alpha(TNF-α)and IL-10 in supernatant were measured using enzyme-linked immunosorbent assay,and the expression of CD86 and NF-κB proteins in microglial cells was detected using Western blot.Results Compared with group C,the BV2 microglial cell viability and concentrations of IL-1βand TNF-αwere significantly increased,the concentrations of IL-10 were decreased,and the expression of CD86 and NF-κB in microglial cells was up-regulated in Mor group(P<0.05).Compared with Mor group,the BV2 microglial cell viability and concentrations of IL-1βand TNF-αwere significantly decreased,the concentrations of IL-10 were increased,and the expression of CD86 and NF-κB in microglial cells was down-regulated in Mor+BBR group(P<0.05).Conclusions BBR can inhibit morphine-induced activation of BV2 microglial cells.
作者
韩帅
史建稳
王梓
肖迎港
梁永新
葛亚丽
高巨
Han Shuai;Shi Jianwen;Wang Zi;Xiao Yinggang;Liang Yongxin;Ge Yali;Gao Ju(Department of Anesthesiology,Northern Jiangsu People′s Hospital,Clinical Medical School,Yangzhou University,Yangzhou 225009,China;Department of Anesthesiology,Women′s and Children′s Hospital Affiliated to Qingdao University,Qingdao 266075,China)
出处
《中华麻醉学杂志》
CSCD
北大核心
2023年第11期1360-1363,共4页
Chinese Journal of Anesthesiology
基金
国家自然科学基金(82172190)
山东省自然科学基金(ZR2023MH270)。
