摘要
目的运用网络药理学筛选芍藤方治疗干燥综合征的活性成分、靶基因及信号通路,并进行相关实验验证,探索芍藤方治疗干燥综合征的作用机制。方法通过检索TCMSP收集芍藤方组方药物的活性成分和作用靶点,通过GeneCards数据库收集干燥综合征的靶基因,运用Venn获得药物与疾病的交集靶点,将交集靶点导入STRING数据库构建PPI网络,采用Cytospace 3.7.2软件构建"药物-活性成分-治疗靶点-疾病"网络,利用DAVID数据库进行GO功能和KEGG通路富集分析。将18只NOD小鼠采用随机数字表法分为模型组、中药组、羟氯喹组,每组6只,另设6只Balb/C小鼠为正常对照组。中药组灌胃芍藤方2.3 g/kg,羟氯喹组灌胃羟氯喹60 mg/kg,模型组和正常对照组灌胃等体积去离子水,1次/d,连续灌胃4周。记录给药期间各组小鼠日常饮水量,采用HE染色观察颌下腺组织的病理变化,采用ELISA法测定血清炎症因子IL-17和TNF-α水平。结果获得芍藤方主要活性成分39个,干燥综合征1062个靶点,得到药物与疾病的交集靶点64个,包括TNF、IL6、NCOA1、AKT1、TP53等。芍藤方治疗干燥综合征靶点主要影响对细菌的反应、细胞对脂质的反应等生物过程,调控TNF-α通路、IL-17信号通路等。实验结果显示,中药组TNF-α、IL-17水平较模型组降低(P<0.05)。结论芍藤方可通过调节IL-17、TNF-α信号通路,抑制炎症,延缓颌下腺损伤,缓解干燥综合征症状。
Objective To screen the active components,target genes and signaling pathways of Shaoteng Decoction in the treatment of Sjogren's syndrome by network pharmacology;To conduct relevant experimental verification to explore the mechanism of action of Shaoteng Decoction in the treatment of Sjogren's syndrome.Methods The active components and targets of Shaoteng Decoction were collected by retrieving TCMSP.The target genes of Sjogren's syndrome were collected through the GeneCards database.The intersection targets of drugs and diseases were obtained by using Venn.The intersection targets were imported into the STRING database to obtain PPI networks,and the"drug-active component-therapeutic target-disease"network was constructed by Cytospace 3.7.2 software.The DAVID database was used for GO function enrichment analysis and KEGG pathway analysis.The 18 NOD mice were divided into model group,TCM group,hydroxychloroquine group,with 6 mice in each group,and 6 Balb/C mice were set as normal control group.TCM group was gavaged with 2.3 g/kg of Shaoteng Decoction,hydroxychloroquine group was gavaged with 60 mg/kg of hydroxychloroquine,and model group and normal control group were gavaged with equal volume of deionized water once a day for 4 consecutive weeks.The daily water intake of mice during the administration period was recorded,the pathological changes of submandibular gland tissue were observed by HE staining,and the levels of serum inflammatory factors IL-17 and TNF-αwere determined by ELISA method.Results 39 main active components of Shaoteng Decoction,1062 targets of Sjogren's syndrome,and 64 targets of drug and disease intersection were obtained,including TNF,IL6,NCOA1,AKT1,TP53,etc.The treatment targets of Sjogren's syndrome mainly affected biological processes such as response to bacterium and cellular response to lipid,and regulated TNF-αpathway and IL-17 signaling pathway.The experimental results showed that the levels of TNF-αand IL-17 in the TCM group were lower than those in the model group(P<0.05).Conclus
作者
庞枫韬
李克嵩
程增玉
唐晓颇
Pang Fengtao;Li Kesong;Cheng Zengyu;Tang Xiaopo(Department of Rheumatology,Guang'anmen Hospital,China Academy of Chinese Medicial Sciences,Beijing100053,China)
出处
《国际中医中药杂志》
2024年第1期63-68,共6页
International Journal of Traditional Chinese Medicine
基金
中央级科研院所公益性基金(ZZ15-XY-PT-11)
中国中医科学院科技创新工程(CI2021A01502)。
关键词
芍藤方
干燥综合征
网络药理学
动物实验
炎症
小鼠
近交NOD
Sjogren's Syndrome
Shaoteng Decoction
Network pharmacology
Animal experimentation
Inflammation
Mice,inbred NOD
