摘要
目的:制备一种含沸石咪唑类骨架材料8(ZIF-8)的复合光交联水凝胶,评价其体外细胞毒性、药物释放能力和抗菌性能。方法:采用水热法合成ZIF-8颗粒,扫描电子显微镜(SEM)观察ZIF-8颗粒微观形态表现。将ZIF-8颗粒以质量分数0.2%的比例与甲基丙烯酸酐化明胶(GelMA)混合获得复合水凝胶GelMA-Z。采用原子吸收光谱法检测各时间点GelMA-Z中锌离子(Zn^(2+))累计释放量。NIH-3T3细胞分为对照组、GelMA组和GelMA-Z组,将GelMA和GelMA-Z与NIH-3T3细胞共培养1、3和7 d,采用CCK-8法检测各组细胞存活率。大肠杆菌(E.coli)和金黄色葡萄球菌(S.aureus)分为对照组、GelMA组和GelMA-Z组,将GelMA和GelMA-Z分别与E.coli和S.aureus共培养6、12和24 h,采用酶标仪检测不同时间点各组细菌活性,采用平板抑菌实验和活/死细菌染色实验检测各组细菌菌落形成情况和活/死细菌染色情况。结果:SEM观察,采用水热法合成的ZIF-8颗粒具有均匀的粒径。原子吸收光谱法检测,GelMA-Z中Zn^(2+)在1 d内呈突释阶段后缓慢释放,7 d左右达到平衡。与对照组比较,1、3和7 d时GelMA组和GelMA-Z组细胞存活率均大于90%,差异无统计学意义(P>0.05)。酶标仪检测菌液活性,与细菌共培养6、12和24 h时,与对照组和GelMA组比较,GelMA-Z组E.coli和S.aureus活性明显降低(P<0.05)。平板抑菌实验,GelMA-Z组中菌液涂布后形成的菌落数明显少于对照组和GelMA组。活/死细菌染色实验,GelMA-Z组存在大量红色荧光染色的死细菌,对照组和GelMA组中则为大量绿色荧光染色的活细菌。结论:负载ZIF-8的GelMA水凝胶可实现原位光交联,具有良好的Zn^(2+)释放能力和抗菌性,是一种可用于感染伤口治疗的新型水凝胶敷料。
objective:To prepare a composite photocrosslinked hydrogel containing zeolite imidazole framework-8(ZIF-8),and to evaluate its in vitro cytotoxicity,drug release capability,and antimicrobial propertie.Methods:The ZIF-8 particles were synthesized by hydrothermal method,and the microstructure characteristic was observed under scanning electron microscope(SEM).The particles were mixed with the gelatin methacryloyl(GelMA)with the mass fraction of 0.2%to obtain the composite hydrogel GelMA-Z.The atomic absorption spectroscope was used to detect the cumulative zinc ion(Zn^(2+))release amounts in GelMA-Z at different time points.The NIH-3T3 cells were co-cultured with GelMA-Z for 1,3,and 7 d;the viabilities of the cells in various groups were detected by CCK-8 assay;the GelMA-Z was co-cultured with Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)for 6,12,and 24 h and divided into control group,GelMA group,and GelMA-Z group.The bacterial activities of the cells in various groups at different time points were detected by microplate reader;the bacterial formation and the presence of live/dead becterial staining condition were detected by plate antibacterial experiment and live/dead bacterial staining method.Results:The SEM observation results showed that the hydrothermally synthesized ZIF-8 particles had the uniform particle sizes.The atomic absorption spectroscope results showed that Zn^(2+)in GelMA-Z showed an initial burst phase within 1 d,followed by a slow release,and reached the equilibrium around 7 d.Compared with control group,the viabilities the cells in GelMA group and GelMA-Z group were above 90%on the 1st,3rd,and 7th days,but there was no significant difference(P>0.05).The bacterial activity detection results showed that when co-cultured with bacteria for 6,12,and 24 h,compared with control group and GelMA group,the bacterial activities of the E.coli and S.aureus in GelMA-Z group were decreased(P<0.05).The plate antibacterial experiment results showed that the number of bacterial formation in GelMA-Z
作者
姜孔昭
李驰宇
罗云纲
刘志辉
JIANG Kongzhao;LI Chiyu;LUO Yungang;LIU Zhihui(Department of Prosthodontics,Stomatology Hospital,Jilin University,Changchun 130021,China;Department of Orthopaedics,Second Hospital,Jilin University,Changchun 130022,China;Department of Stomatology,Jingyue Hospital District,First Hospital,Jilin University,Changchun 130117,China)
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2024年第1期106-112,共7页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅医药健康专项项目(20200708107YY)
吉林省科技厅重点研发项目(20220204124YY)。
