摘要
目的:基于“肺脑相关”中医理论,研究不同免疫状态对流行性感冒“肺脑传变”的影响及麻杏石甘汤的干预作用。方法:(1)设正常对照组、正常外感组、免疫缺陷1组、免疫缺陷2组、免疫缺陷外感1组、免疫缺陷外感2组,免疫缺陷1组、免疫缺陷外感1组按照75 mg/kg隔日腹腔注射环磷酰胺1次;免疫缺陷2组、免疫缺陷外感2组按照75 mg/kg隔日腹腔注射环磷酰胺2次;末次注射后24 h,正常外感组、免疫缺陷外感1、2组小鼠滴鼻感染流感病毒。病毒感染后3 d,检测肺脑指数、肺脑组织病理变化、肺脑组织中流感病毒载量水平及Tnfa mRNA及蛋白表达、脑组织中闭锁小带蛋白-1(Zo1)的mRNA及蛋白表达等指标评估不同免疫状态对流感“肺脑传变”的影响。(2)设正常对照组、模型对照组、奥司他韦21.5 mg/kg组、麻杏石甘汤6.05 g/kg组,除正常对照组外,其余各组小鼠按照75 mg/kg腹腔注射环磷酰胺1次,注射后24 h,滴鼻感染流感病毒建立流感病毒感染模型,经灌胃给予药物或生理盐水3 d后,从肺脑指数、肺脑组织病理变化、肺组织中流感病毒载量水平、肺脑组织中Tnfa mRNA及蛋白表达,脑组织中Zo1 mRNA及蛋白表达综合探讨麻杏石甘汤对流感“肺脑传变”小鼠的保护作用机制。结果:(1)与正常对照组及正常外感组比较,免疫缺陷外感组小鼠肺指数、脑指数明显增加(P<0.05);肺组织、脑组织出现严重病理损伤;肺组织中TNF-α蛋白表达显著上调(P<0.01),脑组织中Tnfa mRNA及蛋白表达明显上调(P<0.05);脑组织海马及皮质中ZO-1蛋白表达显著下调(P<0.01)。(2)与正常对照组比较,模型对照组小鼠肺指数、脑指数显著增加(P<0.01);肺、脑组织出现严重病理损伤;肺组织中流感病毒载量明显增高(P<0.05);肺组织中Tnfa mRNA及蛋白、脑组织中Tnfa mRNA表达显著上调(P<0.01);脑组织中Zo1 mRNA及蛋白表达显著下调(P<0.01);与模型对照组比�
Objective:Based on the"lung-brain correlation"theory in traditional Chinese medicine(TCM),this study aimed to investigate the effect of immune status on the"lung-brain transmission"of influenza and the intervention effect of Maxing Shigan Decoction(MXSGD)(麻杏石甘汤).Methods:①A normal control group,a normal infection group,an immune deficiency 1 group,an immune deficiency 2 group,an immune deficiency with infection 1 group,and an immune deficiency with infection 2 group were set up.The immune deficiency 1 group and immune deficiency with infection 1 group were intraperitoneally injected with cyclophosphamide at a dose of 75 mg/kg every other day.The immune deficiency 2 group and immune deficiency with infection 2 group were intraperitoneally injected with cyclophosphamide at a dose of 75 mg/kg every other day twice.After 24 hours of the last injection,the mice in the normal infection group and immune deficiency with infection 1 and 2 groups were intranasally infected with influenza virus.Three days after viral infection,the lung-brain index,pathological changes in lung and brain tissues,influenza virus load levels,Tnf-αmRNA and protein expression in lung and brain tissues,as well as zonule occluden-1(Zo-1)mRNA and protein expression in brain tissues were evaluated to assess the impact of immune status on the"lung-brain transmission"of influenza.②A normal control group,a model control group,an oseltamivir group(21.5 mg/kg),and a MXSGD group(6.05 g/kg)were set up.Mice,except for those in the normal control group,were intraperitoneally injected with cyclophosphamide at a dose of 75 mg/kg.After 24 hours of injection,the mice were intranasally infected with influenza virus to establish the influenza infection model.After oral administration of MXSGD or saline for 3 days,the lung-brain index,pathological changes in lung and brain tissues,influenza virus load levels in lung tissues,Tnf-αmRNA and protein expression in lung and brain tissues,as well as Zo-1 mRNA and protein expression in brain tissues were comp
作者
张香港
赵澄
臧慧
陈纯静
彭美红
卢芳国
ZHANG Xianggang;ZHAO Cheng;ZANG Hui;CHEN Chunjing;PENG Meihong;LU Fangguo(School of Integrated Chinese and Western Medicine,Hunan University of Chinese Medicine,Changsha 410208;Medical School,Hunan University of Chinese Medicine,Changsha 410208;The First Hospital of Hunan University of Chinese Medicine,Changsha 410021)
出处
《中药药理与临床》
CSCD
北大核心
2023年第10期15-23,共9页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金项目(编号:82074250)
湖南省自然科学基金项目(编号:2020JJ4063)
湖南省研究生科研创新项目(编号:CX20220783)
湖南中医药大学中西医结合学院-明康中锦联合基金项目(编号:2022MKZJ01)
湖南中医药大学研究生创新课题项目(编号:2022CX179)
中西医结合病原生物学湖南省重点实验室开放基金项目(编号:2022KFJJ01)。
关键词
麻杏石甘汤
免疫状态
A型流感病毒
肺脑相关
流感“肺脑传变”
Maxing Shigan Decoction(麻杏石甘汤)
Immune status
Influenza A virus
Lung-brain correlation
Lung-brain transmission of influenza
