摘要
为了了解雷州半岛雷州市规模化家禽养殖场腹泻家禽的细菌感染情况,试验对采集的20余份病料(脾脏、肝脏、心包液)进行病原菌的分离培养、革兰氏染色和镜检、麦康凯和伊红美蓝培养基筛选,对筛选到的病原菌进一步进行生化试验和16S rRNA鉴定确定其种类,最后对病原菌进行药敏试验和动物回归试验。结果表明:从病料中分离出31株大肠杆菌和10株沙门氏菌。分离得到的大肠杆菌对多黏菌素B的敏感率仅为58.0%,对头孢噻肟的敏感率为100%,对其他14种药物的耐药率在67.7%~100%之间;沙门氏菌对环丙沙星、多黏菌素B和头孢噻肟的敏感率分别为50.0%、80.0%和100%,对其他13种药物的耐药率在50.0%~100%之间。大肠杆菌和沙门氏菌均对雏鸭具有致病性,病理解剖肝脏和脾脏肿大,表面分布白色坏死点和坏死灶,病原菌分离呈阳性。说明在雷州半岛雷州市规模化家禽养殖场可选择多黏菌素B和头孢噻肟防治致病性大肠杆菌和沙门氏菌的感染。
To investigate the bacterial infection of diarrhea poultry in large-scale poultry farms in Leizhou city,Leizhou Peninsula,more than 20 samples(spleen,liver,pericardial fluid)were isolated and cultured,examined by Gram staining,screened by McConkey and eosinmethblue medium.Further biochemical tests and 16S rRNA identification were carried out to determine the species of the selected pathogens.Finally,drug susceptibility tests and animal regression tests were carried out.The results showed that 31 strains of E.coli and 10 strains of Salmonella were isolated from the samples.The sensitivity rate of isolated E.coli to polycolistin B was only 58.0%;the sensitivity rate to cefotaxime was 100%,and the resistance rate to other 14 drugs was between 67.7%-100%.The susceptibility rates of Salmonella to ciprofloxacin,polymyxins and cefotaxime were 50.0%,80.0%and 100%,respectively,and the resistance rates to other 13 drugs ranged from 50.0%-100%.Both E.coli and Salmonella were pathogenic to the ducklings.The liver and spleen were enlarged with white necrotic spots and necrotic foci on the surface.In conclusion,polycolistin B and cefotaxime could be selected to control the infection of pathogenic E.coli and salmonella in large-scale poultry farms in Leizhou,Leizhou Peninsula.
作者
温锦芳
周慧
姚秋成
葛叶
WEN Jinfang;ZHOU Hui;YAO Qiucheng;GE Ye(College of Binhai Agricultural,Guangdong Ocean University,Zhanjiang 524088,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2024年第1期59-64,共6页
Heilongjiang Animal Science And veterinary Medicine
基金
广东省科技计划广东省水禽健康养殖重点实验室项目(2017B030301008)
广东海洋大学博士启动项目(R17090)
广东省教育厅特色创新强校项目(230420055
B18115)。
关键词
大肠杆菌
沙门氏菌
生化鉴定
药敏试验
家禽
Escherichia coli
Salmonella
biochemical identification
susceptibility
avian
