摘要
目的研究至宝三鞭丸对学习记忆障碍模型小鼠学习记忆能力的改善作用,探讨其作用机制。方法采用东莨菪碱诱导小鼠学习记忆障碍模型,采用Morris水迷宫实验、跳台实验和避暗实验评价小鼠学习记忆能力。采用TCMSP、TCMID数据库及文献检索至宝三鞭丸组方药物的化学成分,通过ADMETlab 2.0筛选潜在活性成分,利用Swiss Target Prediction数据库进行靶点预测,通过Metascape数据库进行GO功能和KEGG通路富集分析,并构建PPI网络,进行MCODE模块分析。结果与模型组比较,至宝三鞭丸高剂量组小鼠水迷宫实验逃避潜伏期缩短(P<0.05),第四象限停留时间和穿越平台次数增多(P<0.05)。获得至宝三鞭丸组方药物863个活性成分,401个潜在靶点,至宝三鞭丸改善认知障碍的作用通路涉及神经活性配体-受体通路、钙离子信号通路、Ras相关蛋白-A(Rap1)信号通路、PI3K-Akt信号通路、MAPK信号传导通路、5-羟色胺受体通路等。结论高剂量的至宝三鞭丸可改善学习记忆障碍模型小鼠学习记忆能力,其作用机制可能与调节MAPK信号通路等多条神经功能相关的信号通路有关。
Objective This study aims to investigate the effects of Zhibao Sanbian Pills on learning and memory ability and explore its mechanism.Methods Cognitive decline and memory impairment in mice was induced by scopolamine.Morris water maze test,platform jumping test and dark avoidance test were used to evaluate the learning and memory ability of mice.TCMSP,TCMID database and literature search were used to search the chemical components of Zhibao Sanbian Pills.The potential active components were screened through ADMETlab 2.0.The targets were predicted by Swiss Target Prediction.GO function and KEGG pathway enrichment analysis were conducted through the Metascape database,and a PPI network was constructed for MCODE module analysis.Results Compared with the model group,the latency of water maze test in the high-dose group of Zhibao Sanbian Pills was shortened(P<0.05),and the time of platform quadrant and the number of crossing increased(P<0.05).863 active components and 401 potential targets of Zhibao Sanbian Pills were collected.The enrichment analysis of MCODE,GO and KEGG pathways found that the action pathways of Zhibao Sanbian Pills to improve cognitive impairment involve neuroactive ligand receptor pathway,calcium signal pathway,Rap1 signal pathway,PI3K-Akt signal pathway,MAPK signal transduction pathway,5-hydroxytryptamine receptor pathway,etc.Conclusion Zhibao Sanbian Pills have the potential to improve the learning and memory ability of mice,which may be realized through MAPK signaling pathway and other neural related signaling pathways.
作者
王官连
张咏梅
谢冉
刘佳霖
鲍磊
刘传文
王朋倩
杜茂波
Wang Guanlian;Zhang Yongmei;Xie Ran;Liu Jialin;Bao Lei;Liu Chuanwen;Wang Pengqian;Du Maobo(Traditional Chinese Medicine Preparation Center,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Yantai Zhongya Medical Health Wine Co.,Ltd.,Yantai 264000,China)
出处
《国际中医中药杂志》
2023年第12期1523-1531,共9页
International Journal of Traditional Chinese Medicine
基金
中国中医科学院科技创新工程项目(CI2021A04313)。
关键词
至宝三鞭丸
记忆障碍
行为学实验
网络药理学
小鼠
Zhibao Sanbian Pill
Cognitive impairment
Behavioral experiment
Network pharmacology
Mice
