摘要
目的 对新疆阿克苏地区某县一起食用自制臭豆腐发生肉毒中毒事件进行流行病学调查和实验室检测,为预防此类中毒事件提供参考依据。方法 根据《食品安全事故流行病学调查技术指南(2012年版)》进行流行病学调查,采集自制臭豆腐样本、患者粪便样本,依据GB 4789.12—2016《食品安全国家标准食品微生物学检验肉毒梭菌及肉毒毒素检验》检测肉毒毒素及肉毒梭菌,同时结合荧光定量PCR检测毒素基因并通过脉冲场凝胶电泳(PFGE)分子分型进行同源性分析。结果 流行病学调查发现,此次事件符合食源性肉毒中毒的流行病学特点,患者的临床表现符合肉毒毒素中毒特征,对症治疗及时。采集2名患者的粪便标本各1份、患者食用剩余的自制臭豆腐1份,从2名患者粪便样本及臭豆腐样本中均分离到A型肉毒梭菌,荧光定量PCR检测到A型毒素基因,动物试验证实为产A型肉毒毒素,PFGE分子分型证实2名患者和自制臭豆腐中分离的肉毒梭菌同源性100%,提示共同污染源。结论 本次食物中毒是一起因食用被A型肉毒梭菌污染的自制臭豆腐而引起的肉毒中毒事件,建议加强公众食品安全知识的宣传,增强食品安全意识,预防肉毒中毒事件的发生。
Objective To conduct an epidemiological investigation and laboratory test,to identify the cause of a botulism that occurred in a county in Aksu,Xinjiang,after consuming homemade stinky tofu,and to offer guidance for preventing similar incidents in the future.Methods According to the"Technical Guidelines for Epidemiological Investigation of Food Safety Incidents(2012 Edition)",an epidemiological investigation was conducted.Homemade stinky tofu samples and patients’fecal samples were collected.Laboratory testing for botulinum toxin and Clostridium botulinum was performed following GB 4789.12-2016"National Food Safety Standard for Microbiological Examination of Food-Examination of Clostridium botulinum and its Toxin".The toxin genes were detected using fluorescence quantitative PCR,molecular subtyping was conducted using pulsed-field gel electrophoresis(PFGE)for homology analysis.Results Based on epidemiological investigation,it was determined that this incident precisely matched the epidemiological characterics of foodborne botulism.The clinical symptoms exhibited by the affected individuals were consistent with the characteristics of botulinum toxin poisoning,and prompt and appropriate treatment measures were administered.One fecal sample was collected from each of the two patients,and an additional sample of the homemade stinky tofu they consumed was obtained.Laboratory analysis successfully isolated type A Clostridium botulinum from both fecal samples as well as the stinky tofu sample.The presence of type A toxin genes was confirmed using fluorescence-based quantitative PCR,and animal experiments confirmed the production of type A botulinum toxin.PFGE demonstrated 100%genetic similarity between the Clostridium botulinum strains isolated from the two patients and homemade stinky tofu,indicated the same source of contamination.Conclusion This incident of food poisoning was caused by the consumption of homemade stinky tofu contaminated with A-type Clostridium botulinum,leading to an outbreak of botulism.It was st
作者
霍雯钰
马鑫
李方
苏静
林羽佳
袁永和
孟卫卫
HUO Wenyu;MA Xin;LI Fang;SU Jing;LIN Yujia;YUAN Yonghe;MENG Weiwei(School of Public Health,Xinjiang Medical University,Urumqi,Xinjiang 830011,China;不详)
出处
《中国国境卫生检疫杂志》
CAS
2023年第6期567-570,共4页
Chinese Journal of Frontier Health and Quarantine
基金
新疆维吾尔自治区自然科学基金项目(2019D01C086)。
关键词
肉毒梭菌
食物中毒
自制臭豆腐
Clostridium botulinum
Food poisoning
Homemade stinky tofu
