摘要
目的 调查马鞍山市野鼠血吸虫感染情况,为血吸虫病精准防控提供科学依据。方法 2021年9月—2022年11月,根据马鞍山市血吸虫病历史疫情资料,选取近3年环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)检出感染性钉螺的乡镇(街道)的部分重点环境,对其野鼠分布及血吸虫感染情况实施调查。采用夹夜法投放鼠夹,并对捕获野鼠采用肝脏压片镜检、肝脏匀浆镜检、肠系膜静脉压片镜检、Kato-Katz法和毛蚴孵化法进行平行检测,分析野鼠密度和血吸虫感染率等相关指标。结果 此次调查共抽取4个乡镇(街道)的15个重点环境,累计捕获野鼠343只,平均野鼠密度为6.40%(343/5 362)。各乡镇(街道)中,花山区金家庄街道野鼠密度为12.43%(69/555),雨山区佳山乡为9.62%(50/520),当涂县湖阳镇为6.76%(148/2 188),当涂县黄池镇为3.62%(76/2 099),各乡镇(街道)野鼠密度差异有统计学意义(χ~2=70.271,P<0.05)。捕获有效野鼠样本326只,其中黄毛鼠173只、黑线姬鼠122只、褐家鼠23只、小家鼠7只、针毛鼠1只。检出血吸虫感染野鼠36只,分别来自当涂县湖阳镇和黄池镇,两地野鼠感染率分别为18.57%(26/140)和13.16%(10/76),差异无统计学意义(χ~2=1.039,P>0.05)。湖阳镇检出的阳性野鼠种类为黄毛鼠、黑线姬鼠、褐家鼠和小家鼠,感染率分别22.00%(22/100)、6.45%(2/31)、33.33%(1/3)和20.00%(1/5),差异无统计学意义(Fisher确切概率法,P>0.05);黄池镇检出的阳性野鼠种类为黄毛鼠和黑线姬鼠,感染率分别为20.59%(7/34)和13.64%(3/22),差异无统计学意义(χ~2=0.094,P>0.05)。结论 野鼠可能是当前马鞍山市血吸虫病重要传染源之一。今后应继续加强流行区野鼠监测,对重点环境开展灭螺的同时,适时结合灭鼠、环境整治等措施降低野鼠密度,进一步降低血吸虫病传播风险。
Objective To investigate the prevalence of Schistosoma japonicum infection in wild mice in Ma'anshan City,so as to provide a scientific basis for precise control of schistosomiasis.Methods From September 2021 to No-vember 2022,we conducted a field survey based on the historical epidemic data of schistosomiasis in Ma'anshan City in partial key environments in the townships confirmed with presence of infected snails by Loop-mediated isothermal am-plification(LAMP)method in the past three years for the distribution of wild mice and the status of schistosomiasis in-fection.Mousetraps were used to overnight capture the mice.Parallel examinations were conducted on captured wild mice using liver compression microscopy,liver homogenate microscopy,mesenteric vein compression microscopy,Ka-to-Katz method,and miracidium hatching method.Finally,the relevant indicators,including the density of wild mice and Schistosoma japonicum infection rate,were analyzed.Results Fifteen key environments were selected from 4 townships(communities)in this survey,and a total of 343 wild mice were captured.The average density was 6.40%(343/5362)for the wild mice.The density of wild rodents in each township(community)was 12.43%(69/555)in Jinjia-zhuang Community of Huashan District,9.62%(50/520)in Jiashan Town of Yushan District,6.76%(148/2188)in Huy-ang Town,and 3.62%(76/2099)in Huangchi Town of Dangtu County.The overall mouse density was different among the townships(χ2=70.271,P<0.05).Three hundred and twenty-six valid wild mouse samples were obtained,including 173 Rattus losea,122 Apodemus agrarius,23 Rattus norvegicus,7 Mus musculus and 1 Niviventer fulvescens.Thirty-six wild mice were detected to be infected with Schistosoma japonicum.The infection was seen in Huyang Town and Huangchi Town of Dangtu County,with an infection rate of 18.57%(26/140)and 13.16%(10/76),respectively,for the mice captured in the two areas aforementioned,yet the difference was insignificant(χ2=1.039,P>0.05).The wild mouse species in Huyang Town with positive resul
作者
范泽涵
汪为春
詹惕
段永梅
朱应富
项可霞
江小鱼
罗颖
何家昶
操治国
FAN Zehan;WANGWeichun;ZHAN Ti;DUAN Yongmei;ZHU Yingfu;XIANG Kexia;JIANG Xiaoyu;LUO Yin;HE Jiachang;CAO Zhiguo(Ma'anshan Center for Disease Control and Prevention,Ma'anshan 243000,Anhui Province,China;Anhui Provincial Institute of Schistosomiasis Control)
出处
《热带病与寄生虫学》
CAS
2023年第6期349-352,共4页
Journal of Tropical Diseases and Parasitology
基金
安徽省重点研究与开发计划项目(2022e07020003)
马鞍山市医疗卫生领域科技计划项目(YL-2021-29)。
关键词
野鼠
血吸虫
感染率
马鞍山市
Wild mice
Schistosoma japonicum
Infection rate
Ma'anshan City
