摘要
以食用菌玉木耳子实体为原料,采用陶瓷膜分离法获得玉木耳多糖ACP01,并以传统醇沉法分离获得的玉木耳多糖ACP02作为对照,研究玉木耳多糖的分子结构、流变学特性及体外免疫调节活性。研究结果表明:玉木耳多糖是一种主要以1,3-Manp和1,3-Glcp为主链,Xyl、Man和Glc糖苷键为侧链的高支化酸性多糖。此外,相对于ACP02,ACP01具有更高的纯度和更低的分子量。流变学特性上,玉木耳多糖溶液表现出剪切稀化特性,在黏弹性的测量上以弹性特征为主;在尿素溶液中,ACP02的凝胶维持能力比ACP01更稳固。生物活性上,玉木耳多糖显著促进了RAW-Blue~(TM)细胞产生分泌型碱性磷酸酶(secreted alkaline phosphatase,SEAP)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)和一氧化氮(nitric oxide,NO),表明玉木耳多糖能够有效激活巨噬细胞,发挥免疫功能,具有成为理想的免疫调节剂的潜力。
The Auricularia cornea var.Li.polysaccharide ACP01 was separated from the fruiting body by ce‐ramic membrane.Another polysaccharide ACP02,extracted by the alcohol precipitation method,was used as the control.Their molecular structures,rheological properties,and immunomodulatory activities in vitro were investigated.Structurally,ACP01 and ACP02 were highly branched acidic polysaccharideswith 1,3‐Manp and 1,3‐Glcp as the main chain and Xyl,Man and Glc glycosidic linkages as side chains.ACP01 exhibited higher purity and lower molecular weight than ACP02.The solutions of ACP01 and ACP02 demonstrated shear‐thin‐ning behavior,and higher elastic modulusthan viscous modulus.The gel performance of ACP02 was more stable than that of ACP01 in different concentrations of urea solutions.Regarding biological activity,ACP01 and ACP02 significantly enhanced the production of secreted alkaline phosphatase(SEAP),tumor necrosis factor‐α(TNF‐α),interleukin‐6(IL‐6),and nitric oxide(NO)in RAW‐BlueTM cells,demonstrating the abil‐ity to activate macrophages and exert immunomodulatory functions.The results suggested that the polysaccha‐ride from Auricularia cornea var.Li.had the potential to be developed as an ideal immune modulator and anti tumor drug.
作者
李欣雪
岳红霞
郭晓宇
邵严辉
孙振欧
王贺琪
刘海涛
郭庆彬
LI Xinxue;YUE Hongxia;GUO Xiaoyu;SHAO Yanhui;SUN Zhenou;WANG Heqi;LIU Haitao;GUO Qingbin(College of Food Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China)
出处
《食品研究与开发》
CAS
2024年第1期1-10,共10页
Food Research and Development
基金
国家自然科学基金项目(32272270)
天津市科技计划项目(21ZYJDJC00110)。
关键词
玉木耳
多糖
陶瓷膜
分子结构
流变学
免疫活性
Auricularia cornea var.Li.
polysaccharide
ceramic membrane
molecular structure
rheology
immunomodulatory activity
