摘要
通过融合激光诱导荧光原理和光纤传感原理,集成全光纤光学系统、微型化流动进样系统、高灵敏微型光电探测系统和基于智能手机的APP软件,研制了用于水中抗生素现场快速灵敏检测的便携式智能手机赋能荧光生物传感器.以喹诺酮类抗生素诺氟沙星(NOR)为例,在优化条件下,该荧光生物传感器对NOR的检测限可达0.35μg/L,线性检测范围为1.6~20.6µg/L.所建立的NOR检测方法也被用于各种水样的NOR加标回收检测,其回收率为85%~120%,检测时间少于15min,表明其可以用于实际样品中NOR的现场灵敏快速检测.结合物联网等新兴技术,可根据设定的程序将检测结果直接上传环境监测监管中心,为实现水环境安全的监测预警和应急监测提供重要技术支持.
Combining the principle of laser-induced fluorescence and optical fiber biosensing,a portable smartphone powered fluorescence biosensor was constructed for rapid and sensitive on-site detection of antibiotics in waterby integrating an all fiber optical system,a miniaturized flow sampling system,a highly sensitive micro-photoelectric detection system,and a smartphone based APP software.Taking Norfloxacin(NOR),a kind of the quinolone antibiotic,for example,the detection limit of the proposed biosensor was 0.35μg/L and the linear detection range was 1.6~20.6µg/L under optimized conditions.The established NOR detection method was used for the NOR recovery detection in various spiked water samples.The recovery rate of NOR was 85%~120%and a detection period was less than 15min,indicating that this biosensor could be used for rapid and sensitive on-site detection of NOR in actual samples.Combining emerging technologies,such as the Internet of Things,the detection results can be directly uploaded to the environmental monitoring and supervision center according to the set procedures,providing important technology support to achieve early warning and emergency monitoring of water environmental safety.
作者
卓雨欣
徐文娟
程源
龙峰
ZHUO Yu-xin;XU Wen-juan;CHENG Yuan;LONG Feng(School of Environment and Natural Resources,Renmin University of China,Beijing 100872,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2024年第1期482-488,共7页
China Environmental Science
基金
中国人民大学2023年度拔尖创新人才培育资助计划成果。
