摘要
[目的]比较遮荫及环剥处理对红锥(Castanopsis hystrix)幼苗下冠层叶片抗性生理的影响,为了解红锥对遮荫及环剥的响应机制提供依据。[方法]采用二因素随机区组设计,以2年生红锥幼苗为试材,对其活枝冠层1/3以下进行遮荫及环剥处理。分别设置25%遮荫、45%遮荫、70%遮荫和85%遮荫共4种遮荫强度,以未遮荫为对照;并在遮荫与非遮荫交界处进行环剥处理,探讨遮荫及环剥对红锥幼苗下冠层叶片渗透调节物质、保护酶活性及MDA含量的影响。[结果]红锥幼苗下冠层叶片可溶性糖、可溶性蛋白和游离脯氨酸(Pro)含量均随遮荫度增加而降低。过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性则随遮荫度增加呈先升高后下降的趋势。其中,单独遮荫处理下,CAT和SOD活性均在45%遮荫时最高;环剥+遮荫处理下,CAT活性在45%遮荫时最高,SOD活性在25%遮荫时最高。过氧化物酶(POD)活性随遮荫度增加呈先下降后升高的趋势。其中,单独遮荫处理下,POD活性在45%遮荫时最低;环剥+遮荫处理下,在25%遮荫时最低。丙二醛(MDA)含量随遮荫度增加呈先下降后升高的趋势,且单独遮荫及环剥+遮荫处理均在45%遮荫时最低。与未环剥处理相比,环剥处理下红锥下冠层叶片可溶性糖、可溶性蛋白、Pro和MDA含量均有所下降,CAT、POD和SOD活性则有所上升。[结论]红锥幼苗下冠层叶片主要通过协调SOD、POD和CAT活性适应遮荫胁迫;环剥处理下,红锥幼苗下冠层叶片通过调高保护酶活性而降低遮荫胁迫。
[Purpose]Effects of different shading and girdling treatments on resistance physiology of leaves in the lower canopy of Castanopsis hystrix seedlings were compared to understand the response mechanism of C.hystrix to shading and girdling.[Method]Canopy and stem below 1/3 part of 2-year-old C.hystrix seedlings were treated with different shading(25%,45%,70% and 85% shading)and girdling treatments by 2-factor randomized block group design,with unshaded being the control,to investigate the effects of shading and girdling on osmotic regulator,protective enzyme activity and MDA content of leaves in the lower canopy of C.hystrix.[Result]Contents of soluble sugar,soluble protein and proline of leaves in the lower canopy of C.hystrix decreased with enhanced shading treatment.Catalase(CAT)and superoxide dismutase(SOD)activities both increased firstly and then decreased with the increase of shading,while it was the opposite for peroxidase(POD).CAT and SOD activities both peaked at 45% shading under shading treatment solely;under the combined treatments of shading and girdling,CAT and SOD activities peaked at 45% and 25% shading,respectively.Contrarily,POD activity was the lowest at 45% and 25% shading under shading treatment solely and combined treatments,respectively.Malondialdehyde(MDA)content decreased firstly,minimized at 45% shading under solely shading and combined treatment,and then started to rebound.Compared with the treatments without girdling,leaves in the lower canopy of C.hystrix were lower in the contents of soluble sugar,soluble protein,proline and MDA,but higher in the activities of CAT,POD and SOD.[Conclusion]Leaves in the lower canopy of C.hystrix were adaptable to shading stress mainly by coordinated activities of SOD,POD and CAT,in combination of higher protective enzyme activities arisen from girdling.
作者
杨丽萍
张燕
杨康
韦宇
滕维超
谭长强
YANG Liping;ZHANG Yan;YANG Kang;WEI Yu;TENG Weichao;TAN Zhangqiang(Guangxi Forestry Research Institute,Nanning,Guangxi 530002,China;Nanning South Lake Park,Nanning,Guangxi 530021,China;Nanning Rural Revitalization Group Co.,Ltd.,Nanning,Guangxi 530021,China;College of Forestry,Guangxi University,Nanning,Guangxi 530004,China)
出处
《亚热带农业研究》
2023年第4期234-240,共7页
Subtropical Agriculture Research
基金
广西自然科学基金项目“红锥对下冠层枝叶遮阴的响应机制”(2020GXNSFBA297051)
中央引导地方科技发展资金项目“红锥核心种质资源库及共享服务平台建设”(桂科ZY21195049)
广西自筹经费林业科技项目“红锥定向培育集成技术研究”(桂林科研[2022ZC]第31号)。
关键词
抗性生理
局部遮荫
环剥
下冠层
红锥
resistance physiology
partial shading
girdling
lower canopy
Castanopsis hystrix
