摘要
为探究伴生不同作物对主栽番茄根际土壤肥力与健康影响的作用机制,设置番茄伴生生菜、苋菜、菜心、薄荷和葱的栽培模式,基于高通量测序技术分析伴生不同作物栽培模式下主栽番茄根际土壤肥力及细菌、真菌多样性。结果显示:除菜心外,其他4种伴生作物均显著提高了主栽番茄植株根际土壤中涉及氮、磷循环的土壤酶活性以及微生物生物量碳、氮、磷;伴生栽培虽然没有丰富主栽番茄植株根际土壤中细菌、真菌多样性,但改变了主栽番茄植株根际土壤细菌、真菌优势门属间的丰度占比;门分类水平下,伴生管理均提高了变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、Patescibacteria细菌的丰度占比;属分类水平下,norank f Microscillace⁃ae、norank f norank o Vicinamibacterales、芽孢杆菌属(Bacillus)、norank f Gemmatimonadaceae、交替赤杆菌属(Altererythrobacter)细菌是番茄伴生不同作物中丰度占比前10的特有优势细菌属。此外,伴生栽培不同程度地改变了主栽番茄植株根际土壤真菌多样性和丰富度,富集了壶菌门、担子菌门和油壶菌门优势真菌门;同时富集了unclassified o Sordariales、油壶菌属(Olpidium)和锥盖伞属(Conocybe)、Phialemonium、unclassified o Pleosporales、unclassified o Boletales和Sodiomyces属等优势真菌属,亦提高了毛壳菌属(Chaetomium)的丰度占比。研究结果表明,番茄伴生栽培生菜、苋菜、薄荷和葱4种植物均同程度地有助于不提高番茄根际土壤肥力和维护根际微环境土壤健康的作用,其中,伴生栽培生菜和薄荷提升土壤肥力及维护土壤健康的效果优于伴生菜心、苋菜和葱。
The associated cultivation modes of tomato and lettuce,amaranth,cabbage,mint and onion were set up.The effects of different associated crops on the fertility of the rhizosphere soil and the diversity of bacteria and fungi of the main tomato were analyzed based on high-throughput sequencing technology to study the mechanism of the effects of different associated crops on the fertility and health of the rhizosphere soil of the main tomato,and provide theoretical basis and technical support for constructing the ecological cultivation model of tomato.The results showed that the associated cultivation with four associated crops except of cabbage significantly increased the activity of soil enzymes including aminopeptidases and phosphatase,and microbial biomass carbon,nitrogen and phosphorus in the rhizosphere soil of the main tomato plant.Although the associated cultivation did not enrich the microbial diversities in the rhizosphere soil of the main tomato plant,it changed the microbial abundance ratio in the rhizosphere soil of the main tomato plant.At phylum level,the associated cultivation increased the abundance ratio of Proteobacteria,Firmicutes and Patescibactera bacteria.At genus level,norank f.Microscillaceae,norank_f.norank.o.Vicin⁃amibacterales,Bacillus,norank.f.Gemmatimonadaceae,and Altererythrobacter were the top ten unique dominant bacteria in rhizospheres of tomatoes associated with different crops.In addition,the associated cultivation changed the diversity and abundance of fungi in the rhizosphere soil of the main tomato plants to varying degrees,and enriched the dominant fungi of the phylum Chlamydomonas,Basidiomycetes,and Phyllomycetes.At the same time,it enriched the dominant fungi including unclassified o Sordariales,Ol⁃pidium,and Conoxybe,Phallemonium,unclassified o Pleosporales,unclassified o Boletales,and Sodio⁃myces,and increased the abundance ratio of Chaetomium as well.It is indicated that the associated cultivation of lettuce,amaranth,mint and onion in tomato contributes to improve the
作者
杨妲
庞师婵
李祖健
宋静静
杨尚东
YANG Da;PANG Shichan;LI Zujian;SONG Jingjing;YANG Shangdong(College of Agricultue/National Experimental Teaching Demonstration Center of Plant Science,Guangxi University,Nanning 530004,China;Guangxi South Subtropical Agricultural Science Research Insiture,Chongzuo 532415,China;Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation/College of Marine Sciences,Beibu Gulf University,Qinzhou 535011,China)
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2024年第1期22-30,共9页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(31860547)
广西北部湾海洋生物多样性养护重点实验室(北部湾大学)自主基金项目(2019ZC01)
广西学位与研究生教育改革专项(JGY2021013)。
关键词
番茄
伴生栽培
根际
土壤肥力
微生物多样性
tomato(Solanum lycopersicum L.)
associated cultivation
rhizosphere
soil fertility
microbial diversity
