摘要
目的对天名精内生真菌进行分离和鉴定,并从中筛选出具有抗肝癌作用的活性菌株,为寻找新的抗肝癌先导化合物提供菌株资源。方法采用植物组织切块法和平板划线法对天名精内生真菌进行分离纯化;MTT法筛选对肝癌HepG2细胞增殖具有抑制作用的活性菌株,并计算活性显著内生真菌的IC50值;运用形态学鉴定和分子鉴定的方法对活性菌株进行鉴定。结果从天名精中共分离得到60株内生真菌,其中TMJ-03、TMJ-13、TMJ-15、TMJ-23、TMJ-54为活性内生真菌,其发酵物抑制HepG2细胞增殖的IC50值分别为19.49(TMJ-C-03)、31.13(TMJ-C-13)、88.65(TMJ-C-15)、37.42(TMJ-D-23)、17.22(TMJ-D-54)、32.72(TMJ-C-54)μg·mL^(-1)。5株活性菌株均鉴定到种,分别为Aspergillus terreus、Chaetomium globosum、Alternaria tillandsiae、Fusarium proliferatum、Alternaria arborescens。结论首次从天名精中筛选出对HepG2细胞增殖具有抑制作用的活性内生真菌,为寻找新的抗肝癌药物提供了菌株资源。
Objective To isolate and identify endophytic fungi from Carpesium abrotanoides and screen active strains with anti-hepatoma effects,so as to provide strain resources for the development of new anti-hepatoma lead compounds.Methods The endophytic fungi were isolated and purified from Carpesium abrotanoides by plant tissue culture and scribing method,the active strains with inhibitory effects on the proliferation of HepG2 cells were screened by MTT,and the IC50 value of the notably active endophytic fungi was calculated.Morphological and molecular identification were then applied for the identification of active strains.Results Sixty strains of endophytic fungi were isolated from Carpesium abrotanoides,among which,TMJ-03,TMJ-13,TMJ-15,TMJ-23,and TMJ-54 were active.The IC50 values of their fermentation products inhibiting the proliferation of HepG2 cells were 19.49(TMJ-C-03),31.13(TMJ-C-13),88.65(TMJ-C-15),37.42(TMJ-D-23),17.22(TMJ-D-54),and 32.72(TMJ-C-54)μg·mL^(-1),respectively.These five active strains have been identified to species level,namely Aspergillus terreus,Chaetomium globosum,Alternaria tillandsiae,Fusarium proliferatum,and Alternaria arborescens.Conclusion The active endophytic fungi with inhibitory effects on the HepG2 cell proliferation were screened from Carpesium abrotanoides for the first time,which can provide strain resources for the development of new anti-hepatoma drugs.
作者
张艺
姚思凡
孙伍慧
欧阳琳
赵碧清
周小江
ZHANG Yi;YAO Sifan;SUN Wuhui;OUYANG Lin;ZHAO Biqing;ZHOU Xiaojiang(Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;Hunan Engineering Technology Research Center for Standardization and Function of Chinese Herbal Decoction Pieces,Changsha,Hunan 410208,China)
出处
《湖南中医药大学学报》
CAS
2024年第1期47-53,共7页
Journal of Hunan University of Chinese Medicine
基金
湖南省中医药科研计划项目(C2022043,A2022005-4)
湖南省教育厅科学研究项目(19A368)
湖南中医药大学中药学一流学科项目(2018ZYX01)。
关键词
天名精
内生真菌
分离鉴定
抗肝癌
HEPG2细胞
活性菌株
Carpesium abrotanoides
endophytic fungi
isolation and identification
anti-hepatoma
HepG2 cells
active strains
