摘要
目的:运用网络药理学分析独活寄生汤治疗椎间盘退变(intervertebral disc degeneration,IDD)的可能作用机制。方法:在TCMSP平台内检索独活寄生汤的生物活性成分并根据研究需要进行筛选,同时在GeneCards和OMIM数据库中检索椎间盘退变的靶点。运用Cytoscape软件构建“药物成分—靶点—疾病”复杂网络关系图。同时构建韦恩图,得到独活寄生汤治疗IDD的作用靶点。构建蛋白-蛋白相互作用(protein-protein interaction,PPI)网络,使用NetworkAnalyzer进行网络特征分析,并进行基因本体(gene ontology,GO)功能注释和东京基因组百科全书(kyoto gene and genome encyclopedia,KEGG)通路富集分析。结果:共探索出独活寄生汤中的有效活性成分240种,潜在靶点257个,椎间盘退变疾病靶点2 713个,得到共同靶点75个。GO富集分析得到20个条目,KEGG通路富集分析得到20条。结论:独活寄生汤可能是通过调节MAPK3、FOS、RELA、TP53、IL-6、MAPK1、TNF等靶点,调控高级糖基化终末产物-受体(AGE-RAGE)信号通路、TNF信号通路、IL-17信号通路及PI3K-Akt信号通路等来抑制炎症反应、调控细胞凋亡来治疗椎间盘退变。
Objective:To analyze the possible mechanism of action of Dokuto parasite soup in the treatment of intervertebral disc degeneration(IDD)using network pharmacology.Methods:The bioactive components of Dokuto parasite soup were searched in the TCMSP platform and screened according to the research needs,while the targets of intervertebral disc degeneration were searched in the GeneCards and OMIM databases;the“drug component-target-disease”complex network was constructed using Cytoscape software.A complex network of“drug component-target-disease”was constructed using Cytoscape software,and a Wayne diagram was constructed to obtain the targets for IDD treatment with Dokuto parasite soup.Results:A total of 240 active ingredients,257 potential targets,and 2713 targets of intervertebral disc degeneration disease were explored in Dokuto parasite soup,and 75 common targets were obtained.20 entries were obtained from GO enrichment analysis and 20 entries from KEGG pathway enrichment analysis.Conclusion:The herbal formula Dokuto parasite soup may potentially inhibit inflammation and regulate cell apoptosis for the treatment of intervertebral disc degeneration.This is achieved by modulating various targets such as MAPK3,FOS,RELA,TP53,IL-6,MAPK1,TNF,to regulate signaling pathways including advanced glycation end product-receptor for advanced glycation end products(AGE-RAGE),TNF,IL-17,and PI3K-Akt.
作者
陶瑜晶
王想福
谢芋涛
陈伟国
赵恒
张万乾
杨焕莹
杨建霞
任毅
TAO Yu-jing;WANG Xiang-fu;XIE Yu-tao;CHEN Wei-guo;ZHAO Heng;ZHANG Wan-qian;YANG Huan-ying;YANG Jian-xia;REN Yi(Gansu University of Chinese Medicine,Lanzhou,730000,China;Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou,730000,China)
出处
《神经药理学报》
2023年第4期1-7,共7页
Acta Neuropharmacologica
基金
兰州市科技发展计划项目(No.2020-ZD-36)
兰州市人才创新创业项目(No.2020-RC-54)
甘肃省自然科学基金项目(No.21JR1RA058)。
关键词
独活寄生汤
椎间盘退变
作用机制
网络药理学
靶点
信号通路
Dokuto parasite soup
intervertebral disc degeneration
mechanism of action
network pharmacology
targets
signaling pathways
