摘要
试验旨在研究HOXC10基因多态性与清远麻鸡背羽性状的关联性,为适宜优质肉鸡的品种选育提供理论资料。试验以208只清远麻鸡作为研究对象,测定其99日龄时背羽的性状指标,包括背羽长度、重量、羽根长度和羽根直径,利用直接测序法检测HOXC基因第二内含子的单核苷酸多态性(SNP)。结果显示:共筛选出6个SNPs,分别为SNP1(g.865129G>C)、SNP2(g.865204G>T)、SNP3(g.865226C>T)、SNP4(g.866122T>A)、SNP5(g.826133A>G)、SNP6(g.866270C>T)。将不同位点SNPs的基因型与背羽性状进行关联分析发现,SNP1与背羽羽根长度极显著相关(P<0.01),SNP2与背羽羽根长度显著相关(P<0.05),SNP3与背羽羽根直径极显著相关(P<0.01)。研究表明,清远麻鸡HOXC10基因的单核苷酸多态性对背羽性状有显著相关性,可作为鸡背羽性状选育的分子标记。
In order to study the association between the polymorphism of HOXC10 gene and the feather traits of Qingyuan Ma chicken,208 Qingyuan Ma chickens were selected as the research objects.The main wing feather and back wing feather traits were measured at 99 days of age,including the dorsal feather length,weight,feather root length and feather root diameter.The single nucleotide polymorphisms(SNP)of the second intron of HOXC gene were detected by direct sequencing.The results showed that six SNPs were screened,which were SNP1(g.865129G>C),SNP2(g.865204G>T),SNP3(g.865226C>T),SNP4(g.866122T>A),SNP5(g.826133A>G)and SNP6(g.866270C>T).The association analysis between the genotypes of SNP at different loci and feath⁃er traits showed that SNP1 was significantly correlated with the length of main wing feathers and the length of dorsal feather roots(P<0.01),SNP2 was significantly correlated with the length of main wing feathers and the length of dorsal feather roots(P<0.05),and SNP3 was significantly correlated with the diameter of dorsal feather roots(P<0.01).The results indicated that the single nucleotide polymorphism of Qingyuan Ma chicken HOXC10 gene had significant correlation with feather traits,which could be used as a molecular marker for chicken feather traits breeding.
作者
冼远荣
张燕
李达鉴
李丽莹
姜自琴
张德祥
XIAN Yuanrong;ZHANG Yan;LI Dajian;LI Liying;JIANG Ziqin;ZHANG Dexiang(College of Animal Science,South China Agricultural University,Guangzhou,Guangdong 510642;Guangdong Wen's Southern Poultry Breeding Co.,Ltd.,Yunfu,Guangdong 527400)
出处
《中国家禽》
北大核心
2024年第1期109-113,共5页
China Poultry
基金
国家重点研发计划项目(2021YFD1300100)
省级乡村振兴战略专项资金种业振兴项目(2022-XPY-00-004)。
