摘要
目的通过生物信息学方法研究胰高血糖素样肽2受体(glucagon like peptide 2 receptor,GLP2R)在肝癌(Hepatocellular carcinoma,HCC)组织与正常肝脏组织中的差异表达,探寻其在肝癌预后和免疫细胞浸润的作用。方法使用TIMER 2.0数据库探寻GLP2R在各种肿瘤组织及其对应正常组织中的表达差异性;下载TCGA数据库的肝细胞肝癌数据集,使用R软件探究GLP2R在肝癌组织与正常组织中的表达差异性,绘制Kaplan-Meier生存曲线分析GLP2R表达水平与HCC预后的关系;使用R软件对肝癌数据集分析得到差异基因;使用STRING和Cytoscape构建GLP2R与差异基因互作网络,获取关键基因;对GLP2R与关键基因做单因素与多因素Cox回归分析;对GLP2R与关键基因行GO分析富集通路;使用TIMER2.0数据库,探究GLP2R与TIMER、CIBERSORT中免疫细胞浸润的相关性,利用CIBERSORT进一步分析肝癌中浸润免疫细胞的差异性。结果与正常组织相比,肝癌组织GLP2R的mRNA表达水平下调;低表达GLP2R的肝癌患者预后较好,GLP2R可作为肝癌患者不良预后的独立预测因子;GO富集分析显示GLP2R相关基因主要富集在细胞对胰高血糖素刺激的反应等生物过程中;肝癌组织中GLP2R的表达与CD8^(+)T细胞的免疫浸润水平呈负相关。结论GLP2R在肝癌组织中低表达,低表达的GLP2R与肝癌患者较好预后相关,并且影响HCC免疫浸润水平。GLP2R基因可作为HCC潜在的免疫治疗靶点。
Objective To investigate the differential expression of glucagon like peptide 2 receptor(GLP2R)in hepatocellular carcinoma(HCC)tissues and normal liver tissues using bioinformatics methods and to explore its role in prognosis and immune cell infiltration in patients with liver cancer.Methods TIMER2.O database was used to investigate the expression difference of GLP2R in various tumor tissues and its corresponding normal tissues.The hepatocellular carcinoma data sets of TCGA database was downloaded,and R software was used to explore the expression difference of GLP2R in liver cancer tissues and normal tissues.Kaplan-Meier survival curve was drawn to analyze the relationship between GLP2R expression level and HCC prognosis.R software was used to analyze the liver cancer data sets to obtain differential genes.The interaction network between GLP2R and differential genes was constructed using STRING and Cytoscape to obtain key genes.GLP2R and key genes were analyzed by univariate and multivariate Cox regression analysis.The enrichment pathway of GLP2R and key genes was analyzed by GO.TIMER2.O database was used to explore the correlation between GLP2R and immune cell infiltration in TIMER and CIBERSORT.CIBERSORT was used to further analyze the differences of infiltrating immune cells in liver cancer.Results Compared with normal tissues,GLP2R mRNA expression level in liver cancer tissues was down-regulated.Liver cancer patients with low GLP2R expression had better prognosis.GLP2R could be used as an independent predictor of poor prognosis in liver cancer patients:GO enrichment analysis showed that GLP2R-related genes were mainly enriched in biological processes such as cell response to glucagon stimulation.The expression of GLP2R in HCC was negatively correlated with the immunoinfiltration level of CD8^(+)T cells.Conclusions GLP2R expression is low in liver cancer tissues.Low expression of GLP2R is associated with better prognosis of liver cancer patients and affects the level of immune infiltration of HCC.GLP2R gene ca
作者
毛春虎
陈雨恒
陈果
唐浩
伍刚
MAO Chun-hu;CHEN Yu-heng;CHEN Guo;TANG Hao;WU Gang(College of Medicine and Life Science,Chengdu University of Traditional Chinese Medicine,Chengdu 610072,China;Department of Hepatobiliary and Pancreatic Surgery,Sichuan Academy of Medical Sciences&Sichuan Provincial People′s Hospital,Chengdu 610072,China;The First Clinical College of Medicine,Nanjing Medical University,Nanjing 210029,China;Hepatobiliary Center of the First Affiliated Hospital,Nanjing Medical University,Nanjing 210029,China;Faculty of Clinical Medicine,Southwest Medical University,Luzhou 646000,China)
出处
《实用医院临床杂志》
2024年第1期56-62,共7页
Practical Journal of Clinical Medicine
基金
国家自然科学基金青年科学基金项目(编号:81302161)
四川省科技厅应用基础研究项目(编号:2020YJ450)。
关键词
胰高血糖素样肽2受体
GLP2R
肝癌
预后
免疫细胞浸润
Glucagon-like peptide 2 receptor
Hepatocellular carcinoma
Prognosis
Immune cell infiltration
