摘要
目的:研究裂果薯皂苷Ⅰ(SSPHⅠ)对非小细胞肺癌(NSCLC)细胞的生长、迁移和侵袭的影响及其作用机制。方法:利用Autodock软件将SSPHⅠ和关键靶标间质—上皮转化因子(Met)蛋白进行分子对接。采用集落形成法和细胞计数试剂盒(CCK-8)法检测细胞克隆形成能力和细胞的生长曲线,伤口愈合实验和Transwell实验检测细胞的迁移和侵袭能力。用FITC鬼笔环肽荧光染色法检测细胞的肌动蛋白细胞骨架重组,免疫蛋白印迹(western blotting)法检测Met蛋白磷酸化水平。结果:SSPHⅠ明显抑制A549和PC9细胞的克隆能力、细胞活性、迁移和侵袭能力(P<0.05);同时,SSPHⅠ显著抑制F-肌动蛋白细胞骨架形成。分子对接模拟显示SSPHⅠ和Met具有良好的结合活性。western blotting结果显示,SSPHⅠ明显降低了A549细胞的Met蛋白的磷酸化水平。结论:SSPHⅠ可明显抑制NSCLC细胞生长、迁移和侵袭,其机制可能与抑制HGF/Met信号通路有关。
Objective:To investigate the effect of Saponin of Schizocapsa plantaginea HanceⅠ(SSPHⅠ)on the growth,migration,and invasion of non-small cell lung cancer(NSCLC)cells and its mechanism of action.Methods:Molecular docking of SSPHⅠand key target mesenchymal-epithelial transition factor(Met)protein was performed by Autodock software.Colony forming assay and cell counting kit-8(CCK-8)assay were used to detect the cell clone formation ability and cell growth curve.Wound healing assay and Transwell assay were con-formed to detect the cell migration and invasion ability.FITC ghost pen peptide fluorescence staining was used to detect the reorganization of the actin cytoskeleton in cells,and western blotting was applied to detect the phos-phorylation levels of Met protein.Results:SSPHⅠsignificantly inhibited the cloning ability,cell activity,migra-tion and invasion ability of A549 and PC9 cells(P<0.05);meanwhile,SSPHⅠsignificantly inhibited the forma-tion of F-actin cytoskeleton.Molecular docking simulation showed that SSPHⅠand Met had good binding activ-ity.Additionally,the results of western blotting showed that SSPHⅠsignificantly reduced the phosphorylation levels of Met protein in A549 cells.Conclusion:SSPHⅠsignificantly inhibits the growth,migration,and inva-sion of NSCLC cells,and its mechanism may be related to the inhibition of HGF/Met signaling pathway.
作者
周金玲
甘日植
王燕雪
植丽敏
周欢
吕美娴
梁钢
Zhou Jinling;Gan Rizhi;Wang Yanxue;Zhi Limin;Zhou Huan;Lyu Meixian;Liang Gang(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China;College of Pharmacy,Guangxi Medical University,Nanning 530021,China;Guangxi Medical University Cancer Hospital,Nanning 530021,China)
出处
《广西医科大学学报》
CAS
2023年第12期1978-1984,共7页
Journal of Guangxi Medical University
基金
国家自然科学基金资助项目(No.81960737)
2022年广西中医药大学校级科研项目(No.2022QN005)。
关键词
裂果薯皂苷Ⅰ
非小细胞肺癌
生长
侵袭
迁移
间质—上皮转化因子
saponin of schizocapsa plantaginea hanceⅠ
non-small cell lung cancer
growth
invasion
migra-tion
mesenchymal-epithelial transition factor
