摘要
目的 探究子痫前期孕妇血清白细胞相关免疫球蛋白样受体-2(leukocyte-associated immunoglobulin-like receptor-2,LAIR2)、己糖激酶2(hexokinase 2,HK2)水平对子痫前期的诊断价值和对产妇妊娠结局的预测价值。方法 按照巢式病例对照研究的方法选择2020年5月至2022年3月在延安市人民医院产检并分娩的800例孕妇作为观察队列,其中有88例孕妇最终确诊为子痫前期,纳入研究组,根据妊娠结局分为不良组和正常组;并筛选与之年龄、孕周相匹配的60例未发生子痫前期的健康孕妇作为对照组,采用酶联免疫吸附法检测前期留存的各组孕妇血清LAIR2、HK2水平,同时收集各组孕妇的基线资料,比较组间差异;Pearson法分析LAIR2与HK2表达的相关性;绘制受试者工作特征曲线(receiver operating characteristic curve,ROC)分析血清中LAIR2、HK2水平对子痫前期的诊断价值以及对妊娠结局的预测价值。结果 研究组孕妇血清LAIR2、HK2水平显著低于对照组(P<0.05);子痫前期妊娠结局不良组血清LAIR2、HK2水平显著低于正常组(P<0.05);子痫前期孕妇血清LAIR2、HK2水平呈正相关(r=0.248,P<0.05)。血清LAIR2、HK2诊断子痫前期的ROC曲线下面积分别为0.738、0.822,敏感性分别为65.91%、80.68%,特异性均为75.00%,截断值分别为5.22μg/L、24.57 ng/L;血清LAIR2、HK2预测子痫前期孕妇妊娠结局的ROC曲线下面积分别为0.738、0.768,敏感性分别为87.50%、65.62%,特异性分别为55.36%、80.36%,截断值分别为5.00μg/L、20.09 ng/L。结论 子痫前期孕妇血清LAIR2、HK2表达水平均下调,两者表达呈正相关,对子痫前期具有一定的诊断价值,且对子痫前期孕妇的妊娠结局具有一定的预测价值。
Objective To explore the diagnostic value of serum leukocyte-associated immunoglobulin-like receptor-2(LAIR2)and hexokinase 2(HK2)levels for preeclampsia in pregnant women with preeclampsia and their predictive value of maternal pregnancy outcomes.Methods A nested case-control study was conducted to select 800 pregnant women who underwent prenatal examination and delivery at Yan'an People's Hospital from May 2020 to March 2022 as the observation cohort.Among them,88 pregnant women were ultimately diagnosed with preeclampsia and included in the study group.They were divided into adverse group and normal group based on pregnancy outcomes;60 healthy pregnant women who did not develop preeclampsia and matched their age and gestational age were selected as the control group.Enzyme linked immunosorbent assay was used to detect the serum levels of LAIR2 and HK2 in each group of pregnant women retained in the early stage,and baseline data of each group of pregnant women were collected to compare the differences between groups;Pearson's method was used to analyze the correlation between LAIR2 and HK2 expression;Draw receiver operating characteristic curve(ROC)to analyze the diagnostic value of serum LAIR2 and HK2 levels for preeclampsia and their predictive value for pregnancy outcomes.Results The serum levels of LAIR2 and HK2 in the study group were significantly lower than those in the control group(P<0.05).The serum levels of LAIR2 and HK2 in the group with poor pregnancy outcomes in preeclampsia were significantly lower than those in the normal group(P<0.05).The serum levels of LAIR2 and HK2 in pregnant women with preeclampsia were positively correlated(r=0.248,P<0.05).The area under the ROC curve of serum LAIR2 and HK2 in the diagnosis of preeclampsia was 0.738 and 0.822,respectively,the sensitivity was 65.91%and 80.68%,respectively,both had a specificity of 75.00%,and the cut-off value was 5.22μg/L and 24.57 ng/L,respectively;the area under the ROC curve of serum LAIR2 and HK2 for predicting the pregnancy outcome o
作者
郭彩萍
马延玲
GUO Caiping;MA Yaning(Department of Obstetrics and Gynecology,Yan'an People's Hospital,Yan'an 716000,China)
出处
《延安大学学报（医学科学版）》
2023年第4期48-53,共6页
Journal of Yan'an University:Medical Science Edition
