摘要
【目的】了解四川省炉霍县牦牛体表寄生蜱的种类及其斑点热群立克次体(Spotted fever group Rickettsia,SFGR)和无形体的感染情况。【方法】在炉霍县6个乡采集牦牛体表的蜱,经形态学初步鉴定后提取蜱基因组DNA,采用PCR技术分别扩增蜱ITS⁃2、SFGR OmpB和无形体16S rRNA基因部分片段,对阳性产物进行测序、比对及构建进化树,从而确定蜱的种类及其SFGR和无形体的感染情况。【结果】在820份蜱样本中,只包含青海血蜱(Haemaphysalis qinghaiensisus,29/820)和微小扇头蜱(Rhipicephalus micro⁃plus,791/820)。总共有408份蜱样本被检出SFGR,只检出Candidatus Rickettsia longicornii 1种SFGR,总感染率为49.8%(408/820)。本次调查的6个乡均检出SFGR,不同乡之间SFGR感染率的差异极显著(χ^(2)=111.524,P=0.000<0.01)。青海血蜱和微小扇头蜱的SFGR感染率分别为44.8%(13/29)和49.9%(395/791),差异不显著(χ^(2)=0.292,P=0.589>0.5)。有5个乡检出无形体,共检出Anaplasma bovis、A.marginale、A.platys和A.phagocytophilum 4种无形体,总感染率为30.9%(253/820),不同乡之间蜱样本无形体感染率差异极显著(χ^(2)=139.825,P=0.000<0.01)。青海血蜱和微小扇头蜱的无形体感染率分别为6.9%(2/29)和31.7%(251/791),差异极显著(χ^(2)=8.087,P=0.004<0.01)。SFGR与无形体混合感染率为18.8%(154/820)。【结论】炉霍县存在青海血蜱和微小扇头蜱,还携带SFGR和多种无形体,应做好当地蜱传斑点热群立克次体和无形体的防治工作。
【Objective】The present paper aimed to investigate the species of parasitic ticks on the surface of yaks in Luhuo county,Sichuan province,and the infection status of Rickettsia(SFGR)and invisible ticks.【Method】Ticks were collected from the surface of yaks in six vil lages in Luhuo county.After preliminary morphological identification,tick genomic DNA was extracted.PCR technology was used to amplify partial fragments of tick ITS⁃2,SFGR OmpB,and amorphous 16S rRNA genes.The positive products were sequenced,compared,and an ev⁃olutionary tree was constructed to determine the species of ticks and their infection status with SFGR and anaplasma.【Result】A total of 820 adult ticks were collected with only Haemaphysalis qinghaiensisus(29/820)and Rhipicephalus microplus(791/820)found.Rickettsia spp.were detected in all six villages and total infection rate was 49.8%(408/820)with only Candidatus R.longicornii detected.The infec⁃tion rates of SFGR in six villages were statistically significant(χ^(2)=111.524,P=0.000<0.01).The infection rates of SFGR in H.qing⁃haiensisus and R.microplus were 44.8%(13/29)and 49.9%(395/791),respectively with no significant differences(χ^(2)=0.292,P=0.589>0.5).Anaplasma spp.(including A.bovis,A.marginale,A.platys,and A.phagocytophilum)were detected in five villages,with a total infection rate of 30.9%(253/820).The infection rates of Anaplasma in five villages were statistically significant(χ^(2)=139.825,P=0.000<0.01).Also,the infection rates of Anaplasma between H.qinghaiensisus and R.microplus were statistically significant(χ^(2)=8.087,P=0.004<0.01).Co⁃infection of SFGR and Anaplasma was observed with the infection rate of 18.8%(154/820).【Conclusion】There are Qinghai blood tick and micro fanhead tick in Luhuo county,which also carry SFGR and various intangible organisms.Therefore,it is neces⁃sary to do a good job in the prevention and control of local tick borne spotted fever group rickettsia and intangible organisms.
作者
刘鑫
向阳
央金措
秦保亮
肖晨冬
潘瑶
袁东波
郝力力
LIU Xin;XIANG Yang;YANG Jin-cuo;QIN Bao-liang;XIAO Chen-dong;PAN Yao;YUAN Dong-bo;HAO Li-li(College of Animal and Veterinary Sciences,Southwest Minzu University,Chengdu 610041,China;Yanting County High-end Talent Service Center,Yanting,Sichuan 621600,China;Center for Animal Disease Control and Prevention in Luhuo County,Luhuo,Sichuan 626500,China;Center for Animal Disease Control and Prevention in Xinxiang City,Xinxiang,Henan 453000,China;Center for Ani-mal Disease Control and Prevention in Xiangcheng County,Xiangcheng,Sichuan 627850,China;Animal Husbandry Research Institute of Ganzi Tibetan Autonomous Prefecture,Kangding,Sichuan 626000,China;Center for Animal Disease Control and Prevention in Si-chuan Province,Chengdu 610041,China)
出处
《西南农业学报》
CSCD
北大核心
2023年第11期2535-2543,共9页
Southwest China Journal of Agricultural Sciences
基金
西南民族大学研究生创新型科研项目(CX2021SZ65)。
关键词
炉霍县
牦牛
蜱
斑点热群立克次体
无形体
Luhuo county
Yak
Tick
Spotted fever group Rickettsiae
Anaplasma
