摘要
目的 优选低共熔溶剂提取马蹄金总黄酮的工艺。方法 以低共熔溶剂体系摩尔比、含水量、超声功率、超声时间为单因素变量,以马蹄金中黄酮类成分提取率为评价指标,采用Box-Behnken响应面法优选总黄酮提取工艺,并进行验证试验。结果 优选的提取工艺为低共熔溶剂氯化胆碱-乳酸(摩尔比1∶2),含水量为70%,超声(功率为180 W)提取30 min。验证试验结果显示,在此条件下的总黄酮提取率与回归模型预测值的相对误差为0.55%。结论 该优选工艺操作简便、重复性好,可用于马蹄金总黄酮的提取。
Objective To optimize the process of deep eutectic solvents extraction of total flavonoids from Dichondra repens.Methods With the molar ratio,water content,ultrasound power,and ultrasound time of deep eutectic solvent system as single factor variables,and with the extraction rate of flavonoids in Dichondra repens as the evaluation index,the extraction process of the total flavonoid was optimized by the Box-Behnken response surface methodology,and the validation test was conducted.Results The optimal extraction process was as follows:the deep eutectic solvent was choline chloride-lactic acid(molar ratio of 1∶2),with the water content of 70%,and ultrasonic extraction(power of 180 W)for 30 min.The validation test results showed that the relative error between the total flavonoid extraction rate under these conditions and the predicted value of the regression model was 0.55%.Conclusion The optimal process is easy to operate and has good repeatability,which can be used for the extraction of total flavonoids from Dichondra repens.
作者
王志轩
马丽
郭海平
高婧
冯超
周维维
WANG Zhixuan;MA Li;GUO Haiping;GAO Jing;FENG Chao;ZHOU Weiwei(Hebei Yanshan District Genuine Medicinal Herbs Inspection and Testing Technology Innovation Center,Chengde,Hebei,China 067000;Chengde Food and Drug Inspection and Testing Center,Chengde,Hebei,China 067000)
出处
《中国药业》
CAS
2024年第1期45-49,共5页
China Pharmaceuticals
关键词
Box-Behnken响应面法
低共熔溶剂
马蹄金
总黄酮
提取工艺优选
Box-Behnken response surface methodology
deep eutectic solvents
Dichondra repens
total flavonoids
optimization of extraction process
