摘要
目的 探讨邻苯二甲酸单-2-乙基己基酯(mono-(2-ethylhexyl) phthalate,MEHP)对正常肝细胞HL-7702脂肪酸分解代谢相关基因的影响。方法 将HL-7702细胞分别暴露于不同浓度MEHP(0,0.01,1,10,100μmol/L)和油酸(1 mmol/L OA)24 h以及48 h,未暴露MEHP的细胞作为阴性对照组,用MTT实验检测细胞活性,qRT-PCR法检测染毒后细胞内miRNA-34a的转录水平和细胞内脂肪酸分解代谢相关基因(SIRT1、PPARα和CPT1A)表达的转录水平。结果 与阴性对照组相比,油酸组染毒24 h细胞存活率降低(P<0.05),不同浓度MEHP组染毒24 h和48 h细胞存活率没有明显改变(P>0.05)。与阴性对照组相比,染毒24 h,10,100μmol/LMEHP组和油酸组miRNA-34a表达水平明显降低(P<0.05);染毒48 h,0.01μmol/L MEHP组miRNA-34a表达水平明显升高(P<0.05)。与阴性对照组相比,0.01,1,10,100μmol/L MEHP组和油酸组细胞内SIRT1、PPARα和CPT1A mRNA表达水平均无明显变化。结论 不同浓度MEHP均未对肝细胞的细胞活性产生影响,MEHP可降低miRNA-34a表达水平,但是并不能引起脂肪酸分解代谢相关基因mRNA水平改变。
Objective To investigate the effects of mono-(2-ethylhexyl) phthalate(MEHP) on fatty acid catabolism-related genes in HL-7702 hepatocytes.Methods HL-7702 cells were exposed to different concentrations of MEHP(0,0.01,1,10,100 μmol/L)and 1 mmol/L oleic acid(OA) for 24 h and 48 h,respectively.The cells without exposure to MEHP were used as negative control group.The viability of cells was detected by MTT assay,and qRT-PCR was used to detect the transcriptional levels of miRNA-34a and genes(SIRT1,PPARα,CPT1A) related to fatty acid catabolism in cells after exposure.Results Compared with negative control group,the survival rate of cells in OA group decreased after 24 h of exposure(P<0.05),and the survival rate of cells in various concentrations of MEHP groups did not change significantly(P>0.05).Compared with negative control group,the expression levels of miRNA-34a in 10,100 μmol/L MEHP groups and OA group significantly decreased after 24 h of exposure(P<0.05).Compared with negative control group,the miRNA-34a expression level in 0.01 μmol/L MEHP group significantly increased after 48 h of exposure(P<0.05).Compared with negative control group,the mRNA expression levels of SIRT1,PPARα and CPT1A in 0.01,1,10,100 μmol/L MEHP group and OA group showed no significant changes.Conclusion Different concentrations of MEHP have no effect on the cellular viability of hepatocytes.MEHP can reduce the expression level of miRNA-34a,but it does not change the mRNA levels of fatty acid catabolism-related genes.
作者
李肖
李星道
李明虹
陈阳
高文婷
白剑英
LI Xiao;LI Xingdao;LI Minghong;CHEN Yang;GAO Wenting;BAI Jianying(Department of Environmental Health,School of Public Health,Shanxi Medical University,Taiyuan 030001,China)
出处
《山西医科大学学报》
CAS
2023年第12期1572-1576,共5页
Journal of Shanxi Medical University
基金
山西省自然科学基金面上项目(202203021211231)。
