摘要
药品和个人护理品(PPCPs)在水环境中频繁检出,是一类受到国内外环保部门普遍关注的新污染物。准确掌握水环境中PPCPs存在的种类及浓度水平,获取水环境中各类PPCPs的基础污染数据,是环境管理部门进行新污染物管控的重要基础。为此,必须开发准确灵敏、方便快捷且能实现高通量筛查及定量分析的检测方法。本研究采用大体积直接进样-超高效液相色谱-三重四极杆质谱法测定水环境中11大类(抗生素、降压药、降糖药、抗病毒药、β-受体激动剂、硝基咪唑类药物、H2受体拮抗剂、精神麻醉类药物、降血脂药、非甾体抗炎药及其他类药物和杀菌剂类个人护理品)145种PPCPs。水样经0.22μm的再生纤维素滤膜过滤,加入乙二胺四乙酸二钠(Na2EDTA)并调节pH值至6.0~8.0,加入内标混匀后,采用Phenomenex Kinetex C18柱(50 mm×3 mm,2.6μm)进行色谱分离,以含5 mmol/L甲酸铵的0.1%甲酸水溶液-乙腈为正离子扫描模式下的流动相、5 mmol/L甲酸铵水溶液-乙腈为负离子扫描模式下的流动相,分别进行梯度洗脱,质谱智能化分时间段-多反应选择离子监测(Schedule-MRM)模式检测,内标法定量。145种PPCPs在一定的范围内线性良好,方法检出限为0.015~5.515 ng/L,在低、中、高3个加标水平下的回收率为80.4%~128%,相对标准偏差为0.6%~15.6%。将该方法应用于11份地表水样品和6份饮用水样品的检测,结果显示,145种PPCPs中共检出93种化合物,地表水中PPCPs总含量为276.9~2705.7 ng/L,其中抗病毒药、降糖药和精神麻醉类药品的检出率为100%且含量占比最大;饮用水中检出的PPCPs总含量为140.5~211.5 ng/L,主要检出抗生素、抗病毒药和降糖药。该方法简单快捷,可实现上百种PPCPs的同时测定,适用于环境水体中多种PPCPs残留的测定。
Pharmaceuticals and personal care products(PPCPs)are emerging contaminants frequently detected in aquatic environments at trace levels.These chemicals have diverse structures and physicochemical properties and includes pharmaceuticals like antibiotics,antihypertensive drugs,antiviral drugs,and psychotropic drugs that are widely used in large quantities worldwide.Considering the large number of pharmaceuticals currently in usage,it is crucial to establish a priority list of PPCPs that should be monitored and/or treated first.An accurate understanding of the occurrence and levels of PPCPs in aquatic environments is essential for providing objective materials for monitoring these emerging contaminants.Therefore,accurate,efficient,sensitive,and high-throughput screening techniques need to be established for determining and quantifying PPCPs.This study developed a method for the determination of 145 PPCPs(grouped into eleven categories:antibiotics,antihypertensive drugs,antidiabetic drugs,antiviral drugs,β-receptor agonists,nitroimidazoles,H 2 receptor antagonists,psychotropic drugs,hypolipidemic drugs,non-steroidal anti-inflammatory drugs,and others)in water.The method was based on large volume direct injection without sample enrichment and cleanup and used ultra-high performance liquid chromatography-triple quadrupole mass spectrometry(UHPLC-MS/MS).Water samples were collected and filtered through a 0.22-μm regenerated cellulose(RC)filter membrane.Subsequently,Na 2EDTA was added to the samples to adjust their pH to 6.0-8.0.Internal standards were mixed with the solutions,and because of the addition of Na 2EDTA,the interference of metal ions could be eliminated in the determination of compounds,especially for tetracycline and quinolone antibiotics.Among the six filter membranes tested in this study(PES,PFTE-Q,PFTE,MCE,GHP,and RC),RC filter membranes were screened for water sample filtration.The UHPLC-MS/MS parameters were optimized by comparing the results of various mobile phases,as well as by establishing the bes
作者
孙慧婧
张蓓蓓
崔冬妮
董冰洁
王荟
胡冠九
SUN Huijing;ZHANG Beibei;CUI Dongni;DONG Bingjie;WANG Hui;HU Guanjiu(State Environmental Protection Key Laboratory of Monitoring and Analysis for Organic Pollutants in Surface Water,Jiangsu Provincial Environmental Monitoring Center,Nanjing 210019,China;Nanjing Environmental Monitoring Center of Jiangsu Provincial,Nanjing 210019,China)
出处
《色谱》
CSCD
北大核心
2024年第1期24-37,共14页
Chinese Journal of Chromatography
基金
江苏省环境监测科研基金项目计划(1909,2202)
2021年度省级生态环境科研项目(JSZC-G2021-291)。
关键词
超高效液相色谱-三重四极杆质谱
大体积直接进样
药品和个人护理品
环境水体
ultra-high performance liquid chromatography-triple quadrupole mass spectrometry(UHPLC-MS/MS)
large volume direct injection
pharmaceuticals and personal care products(PPCPs)
environmental water
