摘要
为促进通江黑木耳的进一步开发利用,以东北小碗耳、通江黑木耳、白背黑木耳3种不同产地的黑木耳为试材,以提取率和多糖含量为评价指标,利用热水提取法、Sevag脱蛋白得到3种黑木耳多糖,进行Molish反应分析,采用体外测试法考察不同产地黑木耳多糖的抗氧化活性。结果表明,东北小碗耳的多糖提取率和多糖含量最高,分别为2.48%和43.64%,意味着东北小碗耳的多糖提取率和多糖含量存在优势。不同产地黑木耳的多糖有不同的抗氧化活性,其中通江黑木耳多糖在抗氧化活性方面存在较为明显的优势,羟基自由基,1,1-二苯基-2-苦味基肼(DPPH·)自由基,2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS^(+)·)自由基的清除率分别高达50.11%,56.68%和32.64%,还原力能力高达0.12,表明通江黑木耳多糖抗氧化活性方面更具有潜力。
To promote the further development and utilization of Tongjiang Auricularia auricular,three kinds of A.auriculus from different producing areas in Northeast Xiaowan Auricularia,Tongjiang A.auricular and Baibei A.auricular were studied.With extraction rate and polysaccharide content as evaluation index,A.auricular polysaccharides were obtained by hot water extraction and Sevag deproteinization,respectively.The different of A.auricular polysaccharides from different producing areas were investigated by Molish reaction.And the antioxidant activities of polysaccharides from different producing areas were investigated by in vitro.The results showed that the extraction yield and polysaccharides content of Northeast Xiaowan Auricularia were the highest,2.48%and 43.64%,respectively,which indicated that Northeast Xiaowan Auricularia had advantages of extraction rate and polysaccharide content.A.auricular polysaccharides from different producing areas had different antioxidant activities,and Tongjiang A.auricular had obvious advantages in antioxidant activities,the scavenging rates of hydroxyl free radical,DPPH·free radical,and ABTS^(+)·free radical were up to 50.11%,56.68%,32.64%,respectively,the reducing power was as high as 0.12,which indicated that Tongjiang A.uricularia auricular polysaccharides had high antioxidant activities.
作者
曾小彤
王怡
陈立强
张常洪
魏伟
ZENG Xiaotong;WANG Yi;CHEN Liqiang;ZHANG Changhong;WEI Wei(Sichuan Institute of Arts and Science,Dazhou 635000,China)
出处
《黑龙江农业科学》
2024年第1期51-57,共7页
Heilongjiang Agricultural Sciences
基金
特色植物开发研究四川省高校重点实验室2020年开放基金项目(TSZW2002,TSZW2003)。
关键词
黑木耳
多糖
抗氧化活性
通江黑木耳
Auricularia auricular
polysaccharides
antioxidant activities
Tongjiang Auricularia auricular
