摘要
目的:研究虾青素对快速老化小鼠心脏组织形态以及纤维化的影响,并探讨自噬及氧化应激在其中的作用。方法:将8只正常老化小鼠(SAMR1)作为正常对照组。将56只快速老化小鼠(SAMP10)随机分为模型对照组(16只)、虾青素低剂量[0.4 mg/(g·d)]干预组(8只)、虾青素中剂量[0.8 mg/(g·d)]干预组(16只)、虾青素高剂量[1.6 mg/(g·d)]干预组(8只)和虾青素中剂量加3-甲基腺嘌呤(3-MA)抑制剂组(8只)。苏木精-伊红(HE)染色法检测小鼠心脏组织细胞形态改变。Masson染色检测小鼠心肌组织纤维化程度。比色法检测血浆和心脏组织中氧化应激指标丙二醛(MDA)和超氧化物歧化酶(SOD)的水平。蛋白免疫印迹检测各组小鼠心脏组织微管相关蛋白1轻链3(LC3)和自噬效应蛋白(Beclin-1)的表达。结果:与SAMR1组相比,SAMP10模型对照组小鼠心肌细胞纤维疏松、排列紊乱、纤维化严重;血浆和心脏组织MDA水平显著升高、SOD活力显著降低、心脏组织LC3和Beclin-1蛋白表达显著降低(F=23.73、9.43、7.04、14.81、13.73、15.38,均P<0.05)。与SAMP10模型对照组相比,虾青素干预后SAMP10小鼠心肌组织形态及纤维化显著改善;血浆和心脏组织MDA水平显著降低,SOD活力显著升高,心肌组织LC3和Beclin-1蛋白表达均显著增加(均P<0.05),且高剂量改善作用最为明显;与中剂量虾青素干预组相比,中剂量虾青素加3-MA抑制剂组心脏纤维化程度升高,血浆和心脏组织MDA水平升高、SOD活力降低、心脏组织LC3和Beclin-1蛋白表达降低(F=18.75、6.353、15.77、14.08、22.83、8.657,均P<0.05)。结论:虾青素通过增强自噬、降低氧化应激,从而改善SAMP10衰老小鼠心脏组织损害。
Objective:To investigate the effect of astaxanthin on cardiac morphology and fibrosis in rapidly aging mice,and to explore the role of autophagy and oxidative stress.Methods:Eight normal aging mice(SAMR1)were used as normal control group.56 rapidly aging mice(SAMP10)were randomly divided into model control group(16 mice),astaxanthin low-dose[0.4 mg/(g·d)]group(8 mice),astaxanthin medium-dose[0.8 mg/(g·d)]group(16 mice),and astaxanthin high-dose[1.6 mg/(g·d)]group(8 mice)and astaxanthin medium dose plus 3-MA inhibitor group(8 mice).Hematoxylin-eosin(HE)staining was used to detect the morphological changes of mouse heart tissues.Masson staining was used to detect the degree of myocardial fibrosis.The levels of oxidative stress indexes such as malondialdehyde(MDA)and superoxide dismutase(SOD)in plasma and heart tissue were detected by colorimetry.The expressions of microtubule associated protein 1,light chain 3(LC3)and autophagy effector protein 1(Beclin-1)in heart tissue were detected by Western blotting.Results:Compared with SAMR1 group,SAMP10 model control group showed loose cardiomyocyte fibers,disordered arrangement,increased fibroblasts,severe fibrosis,and significantly increased MDA levels in plasma and heart tissue,SOD activity significantly decreased,LC3 and Beclin-1 protein expression in heart tissue were significantly decreased(F=23.73,9.43,7.04,14.81,13.73,15.38,all P<0.05).However,compared with the SAMP10 model control group,the myocardial morphology and fibrosis were significantly improved,and the plasma and cardiac MDA levels were significantly decreased after astaxanthin intervention in SAMP10 mice,SOD activity significantly increased,and LC3 and Beclin-1 protein expression in myocardial tissue were significantly increased(all P<0.05),and the effect of high dose was the most obvious.Compared with the medium-dose astaxanthin group,the degree of cardiac fibrosis in the medium-dose astaxanthin intervention plus 3-MA inhibitor group was increased,MDA levels in plasma and heart tissue were increased,SO
作者
靳可青
刘银玥
王萌
张绪梅
JIN Keqing;LIU Yinyue;WANG Meng;ZHANG Xumei(Department of Nutrition and Food Science,School of Public Health,Tianjin Medical University,Tianjin 300070,China)
出处
《天津医科大学学报》
2024年第1期35-39,共5页
Journal of Tianjin Medical University
基金
汤臣倍健营养科学研究基金(TY0181104)。
