摘要
本文采用超高效液相色谱-质谱联用法(Ultra Performance Liquid Chromatography-Mass Spectrometry-Mass Spectrometry,UPLC-MS/MS)同时测定酱香型白酒原辅料(高粱和大曲)中的5种非挥发性酚酸(原儿茶酸、对香豆酸、绿原酸、咖啡酸和阿魏酸)。结果显示,利用外标法建立的标准曲线线性关系较好,相关系数(R2)均高于0.995,精密度在0.6%~6.3%,回收率在94.2%~108.1%,准确度在-5.8%~8.1%。该方法操作简单、结果稳定性好、检测数据可靠,可用于酱香型白酒原辅料中非挥发性酚酸含量的测定。
Five non-volatile phenolic acids(protocatechuic acid,p-coumaric acid,chlorogenic acid,caffeic acid and ferulic acid)in the raw materials and excipients of Jiangxiangxing Baijiu(sorghum and Daqu)were simultaneously determined by ultra performance liquid chromatography-mass spectrometry-mass spectrometry(UPLC-MS/MS).The results showed that the standard curve established using the external standard method had a good linear relationship,with correlation coefficients(R2)higher than 0.995,precision ranging from 0.6%to 6.3%,recovery ranging from 94.2%to 108.1%,and accuracy ranging from-5.8%to 8.1%.The method is simple,stable and reliable,and can be used to determine the content of nonvolatile phenolic acids in the raw and auxiliary materials of Jiangxiangxing Baijiu.
作者
骆茂香
刘磊
陈雯
陈仁远
LUO Maoxiang;LIU Lei;CHEN Wen;CHEN Renyuan(Guizhou Provincial Liquor Product Quality Inspectionand Testing Institute,Renhuai 564500,China;Moutai Institute,Renhuai 564500,China)
出处
《食品安全导刊》
2023年第27期118-121,125,共5页
China Food Safety Magazine
关键词
超高效液相色谱-质谱联用法
非挥发性酚酸
酱香型白酒
高粱
大曲
ultra performance liquid chromatography-mass spectrometry-mass spectrometry
non-volatile phenolic acids
Jiangxiangxing Baijiu
sorghum
Daqu
