摘要
目的分析化学发光免疫试验(CLIA)初筛人类免疫缺陷病毒(HIV)阳性结果与确证试验结果的符合性,探讨影响初筛假阳性的主要因素。方法收集2013年1月至2022年3月某部队医院HIV检测数据,以238例CLIA法初筛HIV阳性患者为研究对象,分析不同年龄段、性别、就诊类型、S/CO值、不同科室患者的HIV确证结果,探讨引起初筛结果假阳性的主要原因。结果238个初筛阳性样本中有172个样本送确证试验,其中152例为阳性,52例为阴性,5例为不确定。50岁以上的老年人群确证试验阴性率高于≤20岁和21~50岁年龄组患者确证试验阴性率,差异有统计学意义(P<0.05);且女性患者的确证试验阴性率高于男性患者,差异有统计学意义(P<0.05);住院患者的确证试验阴性率高于门诊患者,差异有统计学意义(P<0.05)。初筛试验S/CO<5的患者确证试验阴性率高于S/CO值5~20、21~50和>50组患者的确证试验阴性率,差异有统计学意义(P<0.05),确证试验阴性率随着S/CO值的升高而逐步降低。HIV确证试验阴性的患者分布于多个科室,主要包括骨科/康复科(21.15%)、泌外/肾内科(15.38%)、妇产科(11.54%)和手足外科(9.62%)。结论CLIA法作为HIV初筛试验时,老年患者、S/CO值较低的一些特殊科室的患者更容易出现假阳性结果,相关工作人员可根据实际情况进行合理的判断与解释。
Objective To explore the main factors affecting the false positive results by analyzing the consistency between the results of preliminary screening for human immunodeficiency virus(HIV)by chemiluminescence immunoassay(CLIA)and confirmatory tests.Methods The data on HIV testing results in a certain military hospital of Central Theater Command from January 2013 to March 2022 were collected,with 238 HIV-positive patients who were preliminarily screened by the CLIA method selected as the research objects.The HIV confirmatory results of patients in different age groups,genders,types of visits,S/CO values,and different departments were analyzed to explore the main causes of false positive results in the preliminary screening.Results Out of 238 preliminarily screened positive samples,172 samples were sent for confirmatory tests,of which 152 were positive,52 were negative,and 5 were uncertain.The negative rate of confirmatory tests in the elderly population aged 50 and above was higher than that in patients aged≤20 and 21-50,with statistically significant difference(P<0.05).The negative rate of confirmatory tests in female patients was higher than that in male patients,with statistically significant difference(P<0.05).The negative rate of confirmatory tests in hospitalized patients was higher than that in outpatient patients,with statistically significant difference(P<0.05).The negative rate of confirmatory tests for patients with S/CO<5 in the preliminary screening test was higher than that of patients with S/CO values of 5-20,21-50,and>50,with statistically significant difference(P<0.05).The negative rate of confirmatory tests gradually decreased as the S/CO value increased.Patients with negative HIV in confirmatory test results are distributed in multiple departments,mainly including orthopedics/rehabilitation(21.15%),urology/nephrology(15.38%),obstetrics and gynecology(11.54%),and hand and foot surgery(9.62%).Conclusion When using the CLIA method in the preliminary screening test for HIV,elderly patients and patie
作者
魏菁菁
傅冬梅
汪薇
刘靓
刘莉
WEI Jingjing;FU Dongmei;WANG Wei;LIU Liang;LIU Li(Clinical Laboratory,Hankou Hospital,General Hospital of Central Theater Command,Hubei,Wuhan 430014,China;Department of Disease Prevention and Control,Hankou Hospital,General Hospital of Central Theater Command,Hubei,Wuhan 430014,China)
出处
《中国医药科学》
2023年第23期155-158,194,共5页
China Medicine And Pharmacy
基金
湖北省自然科学基金指导性项目(2022CFC062)。
关键词
人类免疫缺陷病毒
化学发光法
S/CO值
假阳性
Human immunodeficiency virus
Chemiluminescence immunoassay
S/CO value
False positive
