摘要
为了探究草珊瑚叶和根中黄酮差异积累的分子调控机制,该研究以草珊瑚叶和根为材料,采用液相色谱-质谱联用(LC-MS)技术和高通量转录组测序(RNA-seq)技术进行转录组学和代谢组学联合分析,筛选黄酮类差异代谢物和关键差异代谢酶基因,并随机选取其中8个差异表达基因进行qRT-PCR验证。结果表明,通过代谢组学分析发现在草珊瑚叶和根中共获得37个黄酮相关差异代谢物,包括乔松素、根皮苷、柚皮素、山柰酚、无色矢车菊素、5-O-咖啡酰莽草酸等;通过转录组学分析发现草珊瑚叶和根中共获得36条黄酮相关差异基因,包括2条PAL、3条4CL、2条CHS、4条CHI、2条FLS、1条DFR、1条CYP73A、1条CYP75B1、3条PGT1、6条HCT、2条C3′H、1条CCOAOMT、1条ANR、1条LAR、2条3AT、1条BZ1、2条IFTM7、1条CYP81E9。同时,预测了C2H2、bHLH、bZIP等6个转录因子参与调控草珊瑚叶和根中黄酮合成差异积累。qRT-PCR结果显示,随机选取的8个参与黄酮合成的酶基因在草珊瑚叶和根中上下调表达趋势与转录组测序结果一致。该研究初步解析了草珊瑚叶和根中黄酮类成分差异积累的转录调控机制,为进一步阐明其中关键酶基因及相应转录因子对草珊瑚中黄酮类成分积累的调控作用奠定基础。
This study aims to explore the molecular regulation mechanism of the differential accumulation of flavonoids in the leaves and roots of Sarcandra glabra.Liquid chromatography-mass spectrometry(LC-MS) and high-throughput transcriptome sequencing(RNA-seq) were employed to screen out the flavonoid-related differential metabolites and differentially expressed genes(DEGs) encoding key metabolic enzymes.Eight DEGs were randomly selected for qRT-PCR verification.The results showed that a total of 37 flavonoid-related differential metabolites between the leaves and roots of S.glabra were obtained,including pinocembrin,phlorizin,na-ringenin,kaempferol,leucocyanidin,and 5-O-caffeoylshikimic acid.The transcriptome analysis predicted 36 DEGs associated with flavonoids in the leaves and roots of S.glabra,including 2 genes in the PAL pathway,3 genes in the 4CL pathway,2 genes in the CHS pathway,4 genes in the CHI pathway,2 genes in the FLS pathway,1 gene in the DFR pathway,1 gene in the CYP73A pathway,1 gene in the CYP75B1 pathway,3 genes in the PGT1 pathway,6 genes in the HCT pathway,2 genes in the C3′H pathway,1 gene in the CCOAOMT pathway,1 gene in the ANR pathway,1 gene in the LAR pathway,2 genes in the 3AT pathway,1 gene in the BZ1 pathway,2 genes in the IFTM7 pathway,and 1 gene in the CYP81E9 pathway.Six transcription factors,including C2H2,bHLH,and bZIP,were involved in regulating the differential accumulation of flavonoids in the leaves and roots of S.glabra.The qRT-PCR results showed that the up-or down-regulated expression of the 8 randomly selected enzyme genes involved in flavonoid synthesis in the leaves and roots of S.glabra was consistent with the transcriptome sequencing results.This study preliminarily analyzed the transcriptional regulation mechanism of differential accumulation of flavonoids in the leaves and roots of S.glabra,laying a foundation for further elucidating the regulatory effects of key enzyme genes and corresponding transcription factors on the accumulation of flavonoids in S.glabra.
作者
吴迪
张燕燕
林楠
李叶
张家源
魏艺聪
WU Di;ZHANG Yan-yan;LIN Nan;LI Ye;ZHANG Jia-yuan;WEI Yi-cong(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2023年第21期5767-5778,共12页
China Journal of Chinese Materia Medica
基金
福建省自然科学基金面上项目(2020J01732)
中央本级重大增减支项目(2060302)。
关键词
草珊瑚
转录组
代谢组
黄酮代谢途径
Sarcandra glabra
transcriptome
metabolome
flavonoid metabolic pathway
