摘要
动物源性成分鉴别在肉制品掺假检测中具有重要意义。为检测肉及其制品中猪、牛、羊3种动物源性成分,该研究建立基于通用引物的环介导等温扩增(loop-mediated isothermal amplification,LAMP)高分辨熔解曲线(high-resolution melting,HRM)检测方法。该方法对猪、牛、羊DNA的检测灵敏度分别可达0.3 pg/μL、0.03 pg/μL、0.3 pg/μL;通过分析通用引物扩增子的熔解曲线可实现牛肉、羊肉中分别掺入5%和1%猪肉的鉴别,并成功应用于市售样品检测。该方法快速、灵敏、准确度高,可以实现仅用1组LAMP引物区分猪、牛、羊3种动物源性成分,是一种有效的动物源性成分鉴别方法,在肉及肉制品动物源性成分检验的快速检测中具有良好的发展前景。
With the continuous improvement of people′s living standards,the proportion of consumption of meat products has steadily increased,and the frequent occurrence of adulteration issues not only harms the economic interests and health of consumers but also adversely affects meat processing enterprises.Therefore,the identification of animal-derived ingredients is of great significance in the detection of adulteration in meat products.To detect three animal-derived components in meat and its products,a high-resolution melting curve(HRM)detection method based on universal primers was established for LAMP.The sensitivity of this method for detecting DNA from pigs,cows,and sheep could reach 0.3 pg/μL,0.03 pg/μL,and 0.3 pg/μL.By analyzing the melting curve of the universal primer amplicon,the identification of beef and mutton mixed with 5%and 1%of pork could be achieved,and it was successfully applied to the detection of commercially available samples.This method is fast,sensitive,and highly accurate,and can distinguish three animal-derived ingredients from pigs,cows,and sheep using one set of LAMP primers.It is an effective method for identifying animal-derived components and has good development prospects in the rapid detection of animal-derived components in meat and meat products.
作者
代欣
张月绒
澹台玮
李艳妮
徐秦峰
DAI Xin;ZHANG Yuerong;TANTAI Wei;LI Yanni;XU Qinfeng(School of Food Science and Engineering,Shaanxi University of Science and Technology,Xi′an 710021,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2023年第24期235-240,共6页
Food and Fermentation Industries
基金
国家自然科学基金项目(22074085)。
关键词
肉制品掺假
通用引物
环介导等温扩增
高分辨熔解
adulteration of meat products
universal primers
loop-mediated isothermal amplification
high-resolution melting
