摘要
目前根肿病已成为长江流域油菜主要病害之一,严重影响油菜产业发展。为利用分子标记辅助选择技术快速转育抗根肿病、高配合力亲本,本研究以双抗根肿材料18NS(含PbBa8.1和CRb抗病位点)为供体亲本、波里马恢复系37R为轮回亲本,利用分子标记辅助选择的方法,结合田间表型性状综合选择、室内品质分析、配合力测定以及抗性鉴定结果创制双抗根肿病甘蓝型油菜亲本37RR。通过分子标记辅助选择,在BC3F3世代筛选到8个携带PbBa8.1+CRb纯合抗病位点的株系。根肿病田间抗性鉴定结果表明,8个株系在南充和广元两地均表现为免疫抗性。配合力测定结果表明,8个株系配合力由高到低依次为20S200-2>20S199-8>20S200-11>20S205-3>20S200-8>20S199-1>20S199-7>20S205-1。株系20S200-2于2021年定型并命名为37RR,其杂交种1937R参加西南地区冬油菜品种试验9个试验点中8点增产,平均单产3204.00 kg·hm-2,在获得根肿病抗性的同时,产量较原始杂交种南油1937也有所提升。
Currently,clubroot disease has become one of the main diseases of rapeseed in the Yangtze River basin,which seriously affects the development of rapeseed industry.To use molecular marker assisted selection technology to rapidly transfer parents with resistance to root nodules and high compatibility,18NS(carrying with Pb-Ba8.1 and CRb locus)was used as the donor parent for transferring resistant genes into the pol CMS restorer line 37R.Combined with molecular marker-assisted selection,comprehensive selection of field phenotypic traits,quali-ty analysis,combining ability determination,and resistance identification,a new parent 37RR with double resis-tance of brassica rapeseed to clubroot disease was developed.Through molecular marker-assisted selection,8 lines carrying PbBa8.1+CRb locus were screened in BC3F3 generation.The results of field resistance identification of clubroot disease demonstrated that 8 lines showed immune resistance in Nanchong and Guangyuan.Combining abil-ity of 8 lines was 20S200-2>20S199-8>20S200-11>20S205-3>20S200-8>20S199-1>20S199-7>20S205-1 from high to low.The line 20S200-2 was finally identified and designated"37RR"in 2021.Its hybrid 1937R participated in the winter rape variety test in Southwest China,exhibited increased production at 8 points out of 9 test sites,with an average yield of 3204.00 kg•hm-2 per unit area.While gaining resistance to clubroot disease,its yield was also higher than that of the original hybrid Nanyou 1937.
作者
代兵兵
宋稀
余青青
杨玉恒
周锰林
赵昌斌
邓武明
DAI Bing-bing;SONG Xi;YU Qing-qing;YANG Yu-heng;ZHOU Meng-lin;ZHAO Chang-bin;DENG Wu-ming(Nanchong Academy of Agricultural Sciences,Nanchong 637000,China)
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2023年第6期1150-1155,共6页
Chinese Journal of Oil Crop Sciences
基金
四川省重大科技专项项目(2022ZDZX0015)
国家油菜产业技术体系专项(CARS-12)。
