摘要
建立了超声辅助分散液液微萃取结合气相色谱/质谱联用技术分析玫瑰花露香气组分的方法,为玫瑰花露香气组分研究提供技术支撑。考察了样品量、超声辅助萃取时间、萃取剂种类及其用量、分散剂种类及萃取剂与分散剂的体积比等因素对玫瑰花露香气组分萃取效果的影响。选用HP-INNOWAX色谱柱,分散液液微萃取的最优参数为:样品量7.0 mL、超声辅助萃取时间3 min、萃取剂及用量为二氯甲烷200μL、分散剂为乙腈、萃取剂与分散剂的体积比为1∶2。结果表明:玫瑰花露香气组分共鉴定出62种香气成分,其中玫瑰细胞液鉴定出52种、玫瑰花水42种、鲜花花水46种、腌渍花水47种,相对含量较高的香气组分有香茅醇、羟基香茅醇、丁香酚、芳樟醇、α-松油醇、苯乙醇、橙花醇、香叶醇、甲基丁香酚等。玫瑰细胞液和玫瑰花水、鲜花花水和腌渍花水、不同生产厂家的玫瑰花水香气成分在其相对含量和成分组成上均存在差异。超声辅助分散液液微萃取结合气相色谱/质谱联用技术用于玫瑰花露香气组分的测定,具有操作简单、有机溶剂用量少、快捷灵敏等优点,适合大批量样品的高通量分析。
A method for the analysis of aroma components in rose hydrolat by ultrasonic-assisted dispersive liquid-liquid microextraction combined with gas chromatography/mass spectrometry was developed.It provides technical support for the study of aroma components of rose hydrolat.The effects of sample size,ultrasonic-assisted extraction time,extractant type and dosage,dispersant type and the volume ratio of extractant to dispersant on the extraction efficiency of aroma components of rose hydrolat were investigated.Using the HP-INNOWAX column,The optimum parameters of dispersed liquid-liquid microextraction were as follows:sample size 7.0 mL,ultrasonic-assisted extraction time 3 min,extractant and dichloromethane 200μL,dispersant acetonitrile,volume ratio of extractant to dispersant 1∶2.The results showed that 62 kinds of aroma components were identified,including 52 kinds of rose cell fluid,42 kinds of rose water,46 kinds of fresh flower water and 47 kinds of pickled flower water,the aroma components with higher relative content were citronellol,eugenol,linalool,α-terpineol,phenylethanol,Nerol,geraniol,methyl eugenol,etc.There were differences in relative content and component composition of rose cell fluid and rose water,fresh flower water and pickled flower water,and rose water from different manufacturers.Ultrasonic-assisted dispersive liquid-liquid microextraction combined with gas chromatography/mass spectrometry was applied to the determination of the aroma components of rose hydrolat,it is suitable for high throughput analysis of large batch samples.
作者
雷春妮
王波
王新潮
漆珍珍
常进文
甘国华
LEI Chun-ni;WANG Bo;WANG Xin-chao;QI Zhen-zhen;CHANG Jin-wen;GAN Guo-hua(Lanzhou Customs Technology Center,Lanzhou 730010,China;Gansu Oriental Tianrun Rose Industry Company,Lanzhou 730030,China;Gansu Changsheng Rose Development Company,Lanzhou 730304,China)
出处
《天然产物研究与开发》
CAS
CSCD
2023年第12期2073-2081,共9页
Natural Product Research and Development
基金
甘肃省重点研发计划(22YF7NK221)。
关键词
超声辅助分散液液微萃取
玫瑰花水
香气成分
ultrasonic-assisted dispersive liquid-liquid microextraction
rose hydrolat
aroma components
